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Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain

Authors
 Myungjae Song  ;  Jung Min Lim  ;  Seonwoo Min  ;  Jeong-Seok Oh  ;  Dong Young Kim  ;  Jae-Sung Woo  ;  Hiroshi Nishimasu  ;  Sung-Rae Cho  ;  Sungroh Yoon  ;  Hyongbum Henry Kim 
Citation
 NATURE COMMUNICATIONS, Vol.12(1) : 5617, 2021-09 
Journal Title
NATURE COMMUNICATIONS
Issue Date
2021-09
MeSH
Algorithms* ; Amino Acid Sequence ; Binding Sites / genetics ; CRISPR-Cas Systems* ; DNA / genetics ; DNA / metabolism* ; Gene Editing / methods* ; HCT116 Cells ; HEK293 Cells ; Humans ; Models, Genetic* ; Rad51 Recombinase / genetics ; Rad51 Recombinase / metabolism* ; Reproducibility of Results
Abstract
Although prime editing is a promising genome editing method, the efficiency of prime editor 2 (PE2) is often insufficient. Here we generate a more efficient variant of PE2, named hyPE2, by adding the Rad51 DNA-binding domain. When tested at endogenous sites, hyPE2 shows a median of 1.5- or 1.4- fold (range, 0.99- to 2.6-fold) higher efficiencies than PE2; furthermore, at sites where PE2-induced prime editing is very inefficient (efficiency < 1%), hyPE2 enables prime editing with efficiencies ranging from 1.1% to 2.9% at up to 34% of target sequences, potentially facilitating prime editing applications.
Files in This Item:
T202104669.pdf Download
DOI
10.1038/s41467-021-25928-2
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Rehabilitation Medicine (재활의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Advanced Medical Science Research and Education (첨단의과학교육연구단) > Journal Papers
Yonsei Authors
Kim, Hyongbum(김형범) ORCID logo https://orcid.org/0000-0002-4693-738X
Song, Myungjae(송명재)
Lim, Jung Min(임정민) ORCID logo https://orcid.org/0000-0002-0223-5933
Cho, Sung-Rae(조성래) ORCID logo https://orcid.org/0000-0003-1429-2684
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/186799
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