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Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain

DC Field Value Language
dc.contributor.author김형범-
dc.contributor.author송명재-
dc.contributor.author임정민-
dc.contributor.author조성래-
dc.date.accessioned2021-12-28T16:47:28Z-
dc.date.available2021-12-28T16:47:28Z-
dc.date.issued2021-09-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/186799-
dc.description.abstractAlthough prime editing is a promising genome editing method, the efficiency of prime editor 2 (PE2) is often insufficient. Here we generate a more efficient variant of PE2, named hyPE2, by adding the Rad51 DNA-binding domain. When tested at endogenous sites, hyPE2 shows a median of 1.5- or 1.4- fold (range, 0.99- to 2.6-fold) higher efficiencies than PE2; furthermore, at sites where PE2-induced prime editing is very inefficient (efficiency < 1%), hyPE2 enables prime editing with efficiencies ranging from 1.1% to 2.9% at up to 34% of target sequences, potentially facilitating prime editing applications.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherNature Pub. Group-
dc.relation.isPartOfNATURE COMMUNICATIONS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAlgorithms*-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHBinding Sites / genetics-
dc.subject.MESHCRISPR-Cas Systems*-
dc.subject.MESHDNA / genetics-
dc.subject.MESHDNA / metabolism*-
dc.subject.MESHGene Editing / methods*-
dc.subject.MESHHCT116 Cells-
dc.subject.MESHHEK293 Cells-
dc.subject.MESHHumans-
dc.subject.MESHModels, Genetic*-
dc.subject.MESHRad51 Recombinase / genetics-
dc.subject.MESHRad51 Recombinase / metabolism*-
dc.subject.MESHReproducibility of Results-
dc.titleGeneration of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pharmacology (약리학교실)-
dc.contributor.googleauthorMyungjae Song-
dc.contributor.googleauthorJung Min Lim-
dc.contributor.googleauthorSeonwoo Min-
dc.contributor.googleauthorJeong-Seok Oh-
dc.contributor.googleauthorDong Young Kim-
dc.contributor.googleauthorJae-Sung Woo-
dc.contributor.googleauthorHiroshi Nishimasu-
dc.contributor.googleauthorSung-Rae Cho-
dc.contributor.googleauthorSungroh Yoon-
dc.contributor.googleauthorHyongbum Henry Kim-
dc.identifier.doi10.1038/s41467-021-25928-2-
dc.contributor.localIdA01148-
dc.contributor.localIdA05976-
dc.contributor.localIdA04652-
dc.contributor.localIdA03831-
dc.relation.journalcodeJ02293-
dc.identifier.eissn2041-1723-
dc.identifier.pmid34556671-
dc.contributor.alternativeNameKim, Hyongbum-
dc.contributor.affiliatedAuthor김형범-
dc.contributor.affiliatedAuthor송명재-
dc.contributor.affiliatedAuthor임정민-
dc.contributor.affiliatedAuthor조성래-
dc.citation.volume12-
dc.citation.number1-
dc.citation.startPage5617-
dc.identifier.bibliographicCitationNATURE COMMUNICATIONS, Vol.12(1) : 5617, 2021-09-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Rehabilitation Medicine (재활의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Advanced Medical Science Research and Education (첨단의과학교육연구단) > 1. Journal Papers

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