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Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김형범 | - |
dc.contributor.author | 송명재 | - |
dc.contributor.author | 임정민 | - |
dc.contributor.author | 조성래 | - |
dc.date.accessioned | 2021-12-28T16:47:28Z | - |
dc.date.available | 2021-12-28T16:47:28Z | - |
dc.date.issued | 2021-09 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/186799 | - |
dc.description.abstract | Although prime editing is a promising genome editing method, the efficiency of prime editor 2 (PE2) is often insufficient. Here we generate a more efficient variant of PE2, named hyPE2, by adding the Rad51 DNA-binding domain. When tested at endogenous sites, hyPE2 shows a median of 1.5- or 1.4- fold (range, 0.99- to 2.6-fold) higher efficiencies than PE2; furthermore, at sites where PE2-induced prime editing is very inefficient (efficiency < 1%), hyPE2 enables prime editing with efficiencies ranging from 1.1% to 2.9% at up to 34% of target sequences, potentially facilitating prime editing applications. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Nature Pub. Group | - |
dc.relation.isPartOf | NATURE COMMUNICATIONS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Algorithms* | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | Binding Sites / genetics | - |
dc.subject.MESH | CRISPR-Cas Systems* | - |
dc.subject.MESH | DNA / genetics | - |
dc.subject.MESH | DNA / metabolism* | - |
dc.subject.MESH | Gene Editing / methods* | - |
dc.subject.MESH | HCT116 Cells | - |
dc.subject.MESH | HEK293 Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Models, Genetic* | - |
dc.subject.MESH | Rad51 Recombinase / genetics | - |
dc.subject.MESH | Rad51 Recombinase / metabolism* | - |
dc.subject.MESH | Reproducibility of Results | - |
dc.title | Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학교실) | - |
dc.contributor.googleauthor | Myungjae Song | - |
dc.contributor.googleauthor | Jung Min Lim | - |
dc.contributor.googleauthor | Seonwoo Min | - |
dc.contributor.googleauthor | Jeong-Seok Oh | - |
dc.contributor.googleauthor | Dong Young Kim | - |
dc.contributor.googleauthor | Jae-Sung Woo | - |
dc.contributor.googleauthor | Hiroshi Nishimasu | - |
dc.contributor.googleauthor | Sung-Rae Cho | - |
dc.contributor.googleauthor | Sungroh Yoon | - |
dc.contributor.googleauthor | Hyongbum Henry Kim | - |
dc.identifier.doi | 10.1038/s41467-021-25928-2 | - |
dc.contributor.localId | A01148 | - |
dc.contributor.localId | A05976 | - |
dc.contributor.localId | A04652 | - |
dc.contributor.localId | A03831 | - |
dc.relation.journalcode | J02293 | - |
dc.identifier.eissn | 2041-1723 | - |
dc.identifier.pmid | 34556671 | - |
dc.contributor.alternativeName | Kim, Hyongbum | - |
dc.contributor.affiliatedAuthor | 김형범 | - |
dc.contributor.affiliatedAuthor | 송명재 | - |
dc.contributor.affiliatedAuthor | 임정민 | - |
dc.contributor.affiliatedAuthor | 조성래 | - |
dc.citation.volume | 12 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 5617 | - |
dc.identifier.bibliographicCitation | NATURE COMMUNICATIONS, Vol.12(1) : 5617, 2021-09 | - |
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