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Treatment of FGF-2 onstem cells from inflamed dental pulp tissue from human deciduous teeth

Authors
 JC Kim  ;  J-C Park  ;  S-H Kim  ;  G-I Im  ;  B-S Kim  ;  J-B Lee  ;  E-Y Choi  ;  J-S Song  ;  K-S Cho  ;  C-S Kim 
Citation
 ORAL DISEASES, Vol.20(2) : 191-204, 2014 
Journal Title
 ORAL DISEASES 
ISSN
 1354-523X 
Issue Date
2014
MeSH
Cell Differentiation ; Cells, Cultured ; Dental Pulp/cytology* ; Fibroblast Growth Factor 2/pharmacology ; Fibroblast Growth Factor 2/physiology* ; Humans ; Mesenchymal Stromal Cells/cytology* ; Mesenchymal Stromal Cells/drug effects ; Mesenchymal Stromal Cells/physiology* ; Pulpitis/pathology* ; Tooth, Deciduous
Keywords
biomedical engineering ; inflammatory/immune response and modulation ; mesenchymal stem cells ; tissue development and growth
Abstract
OBJECTIVE: The purposes of this study were to isolate and characterize stem cells from inflamed pulp tissue of human functional deciduous teeth (iSHFD) and to evaluate the influence of fibroblastic growth factor-2 (FGF-2) on the regenerative potential. MATERIALS AND METHODS: We successfully isolated mesenchymal stem cells (MSCs) from the inflamed dental pulp tissue of human deciduous teeth and demonstrated that their regenerative potential could be enhanced by the application of FGF-2 (20 ng ml(-1)) during ex vivo expansion. Isolated stem cells expanded in FGF-2 were characterized using a colony-forming assay, proliferation, migration, in vitro differentiation, in vivo ectopic transplantation assay, and gene expression profiling. RESULTS: MSCs isolated from the inflamed pulp tissue of functional deciduous teeth potentially possess the qualities of those from human exfoliated deciduous teeth. FGF-2 applied to iSHFD during expansion enhanced the colony-forming efficiency of these cells, increased their proliferation and migration potential, and reduced their differentiation potential in vitro. However, the ectopic transplantation of iSHFD/FGF-2 in vivo increased the formation of dentin-like material. CONCLUSION: FGF-2 expansion of stem cells from inflamed pulp tissues of human deciduous teeth can be a good source of stem cells for future clinical applications and a novel way of using discarded inflamed tissues.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/odi.12089/abstract
DOI
10.1111/odi.12089
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute for Periodontal Tissue Regeneration (치주조직재생연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sung Whan(김성환)
Kim, Chang Sung(김창성) ORCID logo https://orcid.org/0000-0003-3902-1071
Park, Jung Chul(박정철)
Song, Je Seon(송제선) ORCID logo https://orcid.org/0000-0001-8620-5629
Cho, Kyoo Sung(조규성) ORCID logo https://orcid.org/0000-0002-6777-5287
Choi, Eun Young(최은영)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/98152
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