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Shh signaling is essential for rugae morphogenesis in mice.

Authors
 Jong-Min Lee  ;  Seita Miyazawa  ;  Jeong-Oh Shin  ;  Hyuk-Jae Kwon  ;  Dae-Woon Kang  ;  Byung-Jai Choi  ;  Jae-Ho Lee  ;  Shigeru Kondo  ;  Sung-Won Cho  ;  Han-Sung Jung 
Citation
 HISTOCHEMISTRY AND CELL BIOLOGY, Vol.136(6) : 663-675, 2011 
Journal Title
HISTOCHEMISTRY AND CELL BIOLOGY
ISSN
 0948-6143 
Issue Date
2011
MeSH
Animals ; Antibodies, Monoclonal/pharmacology ; Apoptosis/drug effects ; Cell Proliferation/drug effects ; Cells, Cultured ; Computer Simulation ; Gene Expression Regulation/drug effects ; Hedgehog Proteins/antagonists & inhibitors ; Hedgehog Proteins/genetics ; Hedgehog Proteins/metabolism* ; Humans ; Immunohistochemistry ; In Situ Hybridization ; Male ; Mice ; Microarray Analysis ; Palate/growth & development* ; Real-Time Polymerase Chain Reaction ; Signal Transduction*
Keywords
Rugae patterning ; Wnt ; Shh ; Sostdc1 ; Reaction–diffusion
Abstract
Palatal ridges, or rugae palatinae, are corrugated structures observed in the hard palate region. They are found in most mammalian species, but their number and arrangement are species-specific. Nine palatal rugae are found in the mouse secondary palate. Previous studies have shown that epithelial Shh signaling in the palatal ridge plays an important role during rugae development. Moreover, Wnt family members, including LEF1, play a functional role in orofacial morphogenesis. To explore the function of Shh during rugae development, we utilized the maternal transfer of 5E1 (anti-Shh antibody) to mouse embryos. 5E1 induced abnormal rugae patterning characterized by a spotted shape of palatal ridge rather than a stripe. The expression patterns of Shh and Shh-related genes, Sostdc1, Lef1 and Ptch1, were disrupted following 5E1 injection. Moreover, rugae-specific cell proliferation and inter-rugae-specific apoptosis were affected by inhibition of Shh signaling. We hypothesize that the altered gene expression patterns and the change in molecular events caused by the inhibition of Shh signaling may have induced abnormal rugae patterning. Furthermore, we propose a reaction-diffusion model generated by Wnt, Shh and Sostdc1 signaling. In this study, we show that Sostdc1, a secreted inhibitor of the Wnt pathway, is a downstream target of Shh and hypothesize that the interaction of Wnt, Shh and Sostdc1 is a pivotal mechanism controlling the spatial patterning of palatal rugae.
Full Text
http://link.springer.com/article/10.1007%2Fs00418-011-0870-7
DOI
10.1007/s00418-011-0870-7
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Others (기타) > 1. Journal Papers
Yonsei Authors
Kwon, Hyuk Jae(권혁제)
Shin, Jeong Oh(신정오) ORCID logo https://orcid.org/0000-0002-6935-0936
Lee, Jong Min(이종민) ORCID logo https://orcid.org/0000-0002-9466-7644
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
Cho, Sung Won(조성원) ORCID logo https://orcid.org/0000-0001-7505-9769
Choi, Byung Jai(최병재)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/94586
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