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In vitro and in vivo characteristics of stem cells derived from the periodontal ligament of human deciduous and permanent teeth

Authors
 Je Seon Song  ;  Seong-Oh Kim  ;  Jae-Ho Lee  ;  Chang-Sung Kim  ;  Han-Sung Jung  ;  Heung-Kyu Son  ;  Hyung-Jun Choi  ;  Seung-Hye Kim 
Citation
 Tissue Engineering Part A, Vol.18(19-20) : 2040-2051, 2012 
Journal Title
 Tissue Engineering Part A 
ISSN
 1937-3341 
Issue Date
2012
Abstract
In many studies, adult stem cells have been found in human periodontal ligament (PDL), but in most cases they were found in the permanent teeth. The aim of the present study was to characterize stem cells from the PDL of deciduous teeth (dPDLSCs) and compare them with those from the PDL of permanent teeth (pPDLSCs). Stem cell markers were examined by a flow cytometric analysis. The results of in vitro differentiation into adipogenic and osteogenic lineages were analyzed by histochemical staining and quantitative reverse transcription-polymerase chain reaction (RT-PCR). The results of in vivo transplantation were analyzed by histological staining, immunohistochemical staining, and quantitative RT-PCR. There were no significant differences in the proliferation rate, cell cycle distribution, expressions of stem cell markers such as Stro-1 and CD146, or in vitro differentiation. The pPDLSC transplants made more typical cementum/PDL-like tissues and expressed more cementum/PDL-related genes (CP23 and collagen XII) than did the dPDLSC transplants. Together, these results suggest that pPDLSCs are better candidates for use in reconstructing periodontium.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/91938
DOI
10.1089/ten.tea.2011.0318
Appears in Collections:
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Periodontology (치주과학교실)
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실)
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실)
Yonsei Authors
김성오(Kim, Seong Oh) ; 김승혜(Kim, Seung Hye) ; 김창성(Kim, Chang Sung) ; 손흥규(Son, Heung Kyu) ; 송제선(Song, Je Seon) ; 이제호(Lee, Jae Ho) ; 정한성(Jung, Han Sung) ; 최형준(Choi, Hyung Jun)
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Full Text
http://online.liebertpub.com/doi/full/10.1089/ten.tea.2011.0318
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