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In vitro and in vivo characteristics of stem cells derived from the periodontal ligament of human deciduous and permanent teeth

 Je Seon Song  ;  Seong-Oh Kim  ;  Seung-Hye Kim  ;  Hyung-Jun Choi  ;  Heung-Kyu Son  ;  Han-Sung Jung  ;  Chang-Sung Kim  ;  Jae-Ho Lee 
 Tissue Engineering Part A, Vol.18(19-20) : 2040-2051, 2012 
Journal Title
 Tissue Engineering Part A 
Issue Date
In many studies, adult stem cells have been found in human periodontal ligament (PDL), but in most cases they were found in the permanent teeth. The aim of the present study was to characterize stem cells from the PDL of deciduous teeth (dPDLSCs) and compare them with those from the PDL of permanent teeth (pPDLSCs). Stem cell markers were examined by a flow cytometric analysis. The results of in vitro differentiation into adipogenic and osteogenic lineages were analyzed by histochemical staining and quantitative reverse transcription-polymerase chain reaction (RT-PCR). The results of in vivo transplantation were analyzed by histological staining, immunohistochemical staining, and quantitative RT-PCR. There were no significant differences in the proliferation rate, cell cycle distribution, expressions of stem cell markers such as Stro-1 and CD146, or in vitro differentiation. The pPDLSC transplants made more typical cementum/PDL-like tissues and expressed more cementum/PDL-related genes (CP23 and collagen XII) than did the dPDLSC transplants. Together, these results suggest that pPDLSCs are better candidates for use in reconstructing periodontium.
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2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Seong Oh(김성오) ORCID logo https://orcid.org/0000-0002-8620-1377
Kim, Seung Hye(김승혜)
Kim, Chang Sung(김창성) ORCID logo https://orcid.org/0000-0003-3902-1071
Son, Heung Kyu(손흥규)
Song, Je Seon(송제선) ORCID logo https://orcid.org/0000-0001-8620-5629
Lee, Jae Ho(이제호) ORCID logo https://orcid.org/0000-0002-1556-3485
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
Choi, Hyung Jun(최형준) ORCID logo https://orcid.org/0000-0002-3315-6912
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