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Molecular sensing for biomarkers of invasive cancer cells using localized surface plasmon resonance

Authors
 Yoochan Hong  ;  Minhee Ku  ;  Jin-Suck Suh  ;  Yong-Min Huh  ;  Dae Sung Yoon  ;  Jaemoon Yang 
Citation
 JOURNAL OF BIOMEDICAL OPTICS, Vol.8879 : 88790, 2013 
Journal Title
 JOURNAL OF BIOMEDICAL OPTICS 
ISSN
 1083-3668 
Issue Date
2013
MeSH
Biomarkers, Tumor/analysis* ; Biosensing Techniques/methods* ; Cell Line, Tumor ; Gold/chemistry ; Humans ; Immobilized Proteins/chemistry ; Immobilized Proteins/metabolism ; Matrix Metalloproteinase 14/chemistry ; Matrix Metalloproteinase 14/metabolism ; Nanotubes/chemistry ; Neoplasm Invasiveness ; Proteolysis ; Surface Plasmon Resonance/methods*
Keywords
Biomarkers, Tumor/analysis* ; Biosensing Techniques/methods* ; Cell Line, Tumor ; Gold/chemistry ; Humans ; Immobilized Proteins/chemistry ; Immobilized Proteins/metabolism ; Matrix Metalloproteinase 14/chemistry ; Matrix Metalloproteinase 14/metabolism ; Nanotubes/chemistry ; Neoplasm Invasiveness ; Proteolysis ; Surface Plasmon Resonance/methods*
Abstract
In this study, we describe the development of cancer biomarker-sensitive nanobiosensor based on localized surface plasmon resonance (LSPR) that enabling recognition for proteolytic activity of membrane type 1 matrix metalloproteinase (MT1-MMP) anchored on invasive cancer cells. First of all, we prepared biomarker-detectable substrate based on gold nanorods (GNRs) using nanoparticle adsorption method. The sensitivity of sensing chip was confirmed using various solvents that having different refractive indexes. Subsequently, MT1-MMP-specific cleavable peptide was conjugated onto surface of GNRs and molecular sensing about proteolytic activity was conducted using MT1-MMP and cell lysates. Collectively, we developed biomarker detectable sensor, which allows for the effective detection of proteolytic activity about MT1-MMP extracted from invasive cancer cells. © (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Full Text
http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1699949
DOI
10.1117/12.2018326
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
5. Research Institutes (연구소) > Yonsei Integrative Research Institute for Cerebral & Cardiovascular Disease (뇌심혈관질환융합연구사업단) > 1. Journal Papers
Yonsei Authors
Ku, Min Hee(구민희) ORCID logo https://orcid.org/0000-0002-1674-1474
Suh, Jin Suck(서진석) ORCID logo https://orcid.org/0000-0001-9455-9240
Yang, Jae Moon(양재문) ORCID logo https://orcid.org/0000-0001-7365-0395
Huh, Yong Min(허용민) ORCID logo https://orcid.org/0000-0002-9831-4475
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/88476
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