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Recording of elapsed time and temporal information about biological events using Cas9

 Jihye Park  ;  Jung Min Lim  ;  Inkyung Jung  ;  Seok-Jae Heo  ;  Jinman Park  ;  Yoojin Chang  ;  Hui Kwon Kim  ;  Dongmin Jung  ;  Ji Hea Yu  ;  Seonwoo Min  ;  Sungroh Yoon  ;  Sung-Rae Cho  ;  Taeyoung Park  ;  Hyongbum Henry Kim 
 CELL, Vol.184(4) : 1047-1063.e23, 2021-02 
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CRISPR ; Cas9 ; DNA ; biological events ; clock ; elapsed time ; exponential decay ; indel ; temporal information ; time ; timer
DNA has not been utilized to record temporal information, although DNA has been used to record biological information and to compute mathematical problems. Here, we found that indel generation by Cas9 and guide RNA can occur at steady rates, in contrast to typical dynamic biological reactions, and the accumulated indel frequency can be a function of time. By measuring indel frequencies, we developed a method for recording and measuring absolute time periods over hours to weeks in mammalian cells. These time-recordings were conducted in several cell types, with different promoters and delivery vectors for Cas9, and in both cultured cells and cells of living mice. As applications, we recorded the duration of chemical exposure and the lengths of elapsed time since the onset of biological events (e.g., heat exposure and inflammation). We propose that our systems could serve as synthetic "DNA clocks."
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1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Others (기타) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Biomedical Systems Informatics (의생명시스템정보학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Rehabilitation Medicine (재활의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyongbum(김형범) ORCID logo https://orcid.org/0000-0002-4693-738X
Kim, Hui Kwon(김희권)
Lim, Jung Min(임정민) ORCID logo https://orcid.org/0000-0002-0223-5933
Jung, Inkyung(정인경) ORCID logo https://orcid.org/0000-0003-3780-3213
Cho, Sung-Rae(조성래) ORCID logo https://orcid.org/0000-0003-1429-2684
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