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Molecular recognition of protecolytic activity in metastatic cancer cells using fluorogenic gold nanoprobes

 Yoochan Hong  ;  Minhee Ku  ;  Dan Heo  ;  Seungyeon Hwang  ;  Eugene Lee  ;  Joseph Park  ;  Jihye Choi  ;  Hyeon Jung Lee  ;  Miran Seo  ;  Eun Jig Lee  ;  Jong In Yook  ;  Seungjoo Haam  ;  Yong-Min Huh  ;  Dae Sung Yoon  ;  Jin-Suck Suh  ;  Jaemoon Yang 
 Biosensors & Bioelectronics, Vol.15(57) : 171-178, 2014 
Journal Title
 Biosensors & Bioelectronics 
Issue Date
We describe the development of biomarker-sensitive nanoprobes based on nanoparticle surface energy transfer (NSET) effect that enabling recognition of the expression of membrane type-1 matrix metalloproteinase (MT1-MMP) anchored on invasive cancer cells and its proteolytic activity simultaneously. First of all, we confirmed invasiveness of cancer cell lines (HT1080 and MCF7) via migration and invasion assay. We also prepared gold nanoparticle (GNP) acts as a quencher for fluorescein isothiocyanate (FITC). This FITC is conjugated in end-terminal of activatable fluorogenic peptide (ActFP). The ActFP attach to surface of GNP (GNP-ActFP) for a targeting moiety and proteolytic activity ligand toward MT1-MMP. The GNP-ActFP can generate fluorescence signal when ActFP is cleaved by proteolytic activity after targeting toward MT1-MMP. In order to study specificity for MT1-MMP, GNP-ActFP is treated to HT1080 and MCF7 cells, and then, we determine the in vitro targeting potential and fluorogenic activity of GNP-ActFP for MT1-MMP via fluorescence multi-reader. We also confirmed fluorogenic activity of GNP-ActFP via confocal microscopic imaging, and finally, endocytosis of GNP-ActFP is observed via cellular transmission electron microscopic imaging.
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5. Research Institutes (연구소) > Yonsei Integrative Research Institute for Cerebral & Cardiovascular Disease (뇌심혈관질환융합연구사업단) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
Yonsei Authors
구민희(Ku, Min Hee) ORCID logo https://orcid.org/0000-0002-1674-1474
서미란(Seo, Mi Ran)
서진석(Suh, Jin Suck) ORCID logo https://orcid.org/0000-0001-9455-9240
양재문(Yang, Jae Moon) ORCID logo https://orcid.org/0000-0001-7365-0395
육종인(Yook, Jong In) ORCID logo https://orcid.org/0000-0002-7318-6112
이유진(Lee, Eu Gene)
이은직(Lee, Eun Jig) ORCID logo https://orcid.org/0000-0002-9876-8370
이현정(Lee, Hyeon Jeong) ORCID logo https://orcid.org/0000-0003-0635-5454
허단(Heo, Dan)
허용민(Huh, Yong Min) ORCID logo https://orcid.org/0000-0002-9831-4475
황승연(Hwang, Seung Yeon)
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