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Molecular recognition of protecolytic activity in metastatic cancer cells using fluorogenic gold nanoprobes

 Yoochan Hong  ;  Minhee Ku  ;  Dan Heo  ;  Seungyeon Hwang  ;  Eugene Lee  ;  Joseph Park  ;  Jihye Choi  ;  Hyeon Jung Lee  ;  Miran Seo  ;  Eun Jig Lee  ;  Jong In Yook  ;  Seungjoo Haam  ;  Yong-Min Huh  ;  Dae Sung Yoon  ;  Jin-Suck Suh  ;  Jaemoon Yang 
 BIOSENSORS & BIOELECTRONICS, Vol.15(57) : 171-178, 2014 
Journal Title
Issue Date
Biosensing Techniques/methods* ; Cell Line, Tumor ; Cell Movement ; Fluorescence ; Fluorescent Dyes*/chemistry ; Gold*/chemistry ; Humans ; MCF-7 Cells ; Matrix Metalloproteinase 14/analysis* ; Matrix Metalloproteinase 14/metabolism ; Nanoparticles*/chemistry ; Nanoparticles*/ultrastructure ; Neoplasm Metastasis/diagnosis* ; Neoplasm Metastasis/pathology ; Neoplasms/diagnosis ; Neoplasms/enzymology ; Neoplasms/pathology* ; Optical Imaging/methods ; Proteolysis
Cancer metastasis ; Fluorescence ; Membrane type 1-matrix metalloproteinase (MT1-MMP) ; Nanoparticle surface energy transfer (NSET) ; Nanoprobe ; Proteolysis
We describe the development of biomarker-sensitive nanoprobes based on nanoparticle surface energy transfer (NSET) effect that enabling recognition of the expression of membrane type-1 matrix metalloproteinase (MT1-MMP) anchored on invasive cancer cells and its proteolytic activity simultaneously. First of all, we confirmed invasiveness of cancer cell lines (HT1080 and MCF7) via migration and invasion assay. We also prepared gold nanoparticle (GNP) acts as a quencher for fluorescein isothiocyanate (FITC). This FITC is conjugated in end-terminal of activatable fluorogenic peptide (ActFP). The ActFP attach to surface of GNP (GNP-ActFP) for a targeting moiety and proteolytic activity ligand toward MT1-MMP. The GNP-ActFP can generate fluorescence signal when ActFP is cleaved by proteolytic activity after targeting toward MT1-MMP. In order to study specificity for MT1-MMP, GNP-ActFP is treated to HT1080 and MCF7 cells, and then, we determine the in vitro targeting potential and fluorogenic activity of GNP-ActFP for MT1-MMP via fluorescence multi-reader. We also confirmed fluorogenic activity of GNP-ActFP via confocal microscopic imaging, and finally, endocytosis of GNP-ActFP is observed via cellular transmission electron microscopic imaging.
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Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
Yonsei Authors
Ku, Min Hee(구민희) ORCID logo https://orcid.org/0000-0002-1674-1474
Seo, Mi Ran(서미란)
Suh, Jin Suck(서진석) ORCID logo https://orcid.org/0000-0001-9455-9240
Yang, Jae Moon(양재문) ORCID logo https://orcid.org/0000-0001-7365-0395
Yook, Jong In(육종인) ORCID logo https://orcid.org/0000-0002-7318-6112
Lee, Eu Gene(이유진)
Lee, Eun Jig(이은직) ORCID logo https://orcid.org/0000-0002-9876-8370
Lee, Hyeon Jeong(이현정) ORCID logo https://orcid.org/0000-0003-0635-5454
Heo, Dan(허단)
Huh, Yong Min(허용민) ORCID logo https://orcid.org/0000-0002-9831-4475
Hwang, Seung Yeon(황승연)
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