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The effect of epigallocatechin-3-gallate (EGCG) on human alveolar bone cells both in vitro and in vivo

 Yon-Joo Mah  ;  Je Seon Song  ;  Seong-Oh Kim  ;  Jae-Ho Lee  ;  Mijeong Jeon  ;  Ui-Won Jung  ;  Seok Jun Moon  ;  Jeong-Hee Kim  ;  Hyung-Jun Choi 
 ARCHIVES OF ORAL BIOLOGY, Vol.59(5) : 539-549, 2014 
Journal Title
Issue Date
Alkaline Phosphatase/metabolism ; Alveolar Process/cytology* ; Animals ; Apoptosis/drug effects ; Calcium Phosphates/pharmacology ; Catechin/analogs & derivatives* ; Catechin/pharmacology ; Cell Movement/drug effects ; Cell Proliferation/drug effects ; Child ; Female ; Flow Cytometry ; Gene Expression ; Humans ; In Vitro Techniques ; Male ; Mice ; Osteogenesis/drug effects* ; Reverse Transcriptase Polymerase Chain Reaction
Alveolar bone-derived cells ; Epigallocatechin-3-gallate (EGCG) ; In vivo transplantation ; Osteogenic differentiation
OBJECTIVE: The effects of epigallocatechin-3-gallate (EGCG), a major catechin in green tea, on human and mouse osteoblasts remain controversial. This study investigated the direct effects of EGCG on human alveolar bone-derived cells (hABCs) both in vitro and in vivo. DESIGN: hABCs which were collected from eight children (aged 7-9 years, seven males and one female) were treated with EGCG at various concentrations (1, 5, 10, 25, and 50μM), and a proliferation assay, flow cytometric analysis for apoptosis evaluation, migration assay, and in vitro osteogenic differentiation were performed. hABCs that were pretreated with 10μM EGCG and mixed with calcium phosphate carrier combined with EGCG (0.1, 0.5, or 1.5mg) in vivo were transplanted into immunodeficient mouse. Histological staining, quantitative gene expressions, and alkaline phosphatase activity were evaluated in the retrieved transplants. RESULTS: The proliferation and migration were decreased when EGCG was present at over 25μM. The osteogenic differentiation increased slightly when EGCG was present at up to 10μM, and clearly decreased for higher concentrations of EGCG. In vivo, the potential for hard-tissue formation was slightly higher for the group with 0.1mg of EGCG than for the control group, and decreased sharply for higher concentrations of EGCG. CONCLUSION: The present observations suggest that EGCG at a low concentration can slightly enhance the osteogenic effect in vivo, whereas at a higher concentration it can prevent the osteogenic differentiation of hABCs both in vitro and in vivo.
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Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Oral Science Research Center (구강과학연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Seong Oh(김성오) ORCID logo https://orcid.org/0000-0002-8620-1377
Moon, Seok Jun(문석준) ORCID logo https://orcid.org/0000-0001-7282-2888
Song, Je Seon(송제선) ORCID logo https://orcid.org/0000-0001-8620-5629
Lee, Jae Ho(이제호) ORCID logo https://orcid.org/0000-0002-1556-3485
Jeon, Mi Jeong(전미정)
Jung, Ui Won(정의원) ORCID logo https://orcid.org/0000-0001-6371-4172
Choi, Hyung Jun(최형준) ORCID logo https://orcid.org/0000-0002-3315-6912
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