Cited 37 times in
The effect of epigallocatechin-3-gallate (EGCG) on human alveolar bone cells both in vitro and in vivo
DC Field | Value | Language |
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dc.contributor.author | 송제선 | - |
dc.contributor.author | 이제호 | - |
dc.contributor.author | 전미정 | - |
dc.contributor.author | 정의원 | - |
dc.contributor.author | 최형준 | - |
dc.contributor.author | 김성오 | - |
dc.contributor.author | 문석준 | - |
dc.date.accessioned | 2015-01-06T16:42:30Z | - |
dc.date.available | 2015-01-06T16:42:30Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0003-9969 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/98612 | - |
dc.description.abstract | OBJECTIVE: The effects of epigallocatechin-3-gallate (EGCG), a major catechin in green tea, on human and mouse osteoblasts remain controversial. This study investigated the direct effects of EGCG on human alveolar bone-derived cells (hABCs) both in vitro and in vivo. DESIGN: hABCs which were collected from eight children (aged 7-9 years, seven males and one female) were treated with EGCG at various concentrations (1, 5, 10, 25, and 50μM), and a proliferation assay, flow cytometric analysis for apoptosis evaluation, migration assay, and in vitro osteogenic differentiation were performed. hABCs that were pretreated with 10μM EGCG and mixed with calcium phosphate carrier combined with EGCG (0.1, 0.5, or 1.5mg) in vivo were transplanted into immunodeficient mouse. Histological staining, quantitative gene expressions, and alkaline phosphatase activity were evaluated in the retrieved transplants. RESULTS: The proliferation and migration were decreased when EGCG was present at over 25μM. The osteogenic differentiation increased slightly when EGCG was present at up to 10μM, and clearly decreased for higher concentrations of EGCG. In vivo, the potential for hard-tissue formation was slightly higher for the group with 0.1mg of EGCG than for the control group, and decreased sharply for higher concentrations of EGCG. CONCLUSION: The present observations suggest that EGCG at a low concentration can slightly enhance the osteogenic effect in vivo, whereas at a higher concentration it can prevent the osteogenic differentiation of hABCs both in vitro and in vivo. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 539~549 | - |
dc.relation.isPartOf | ARCHIVES OF ORAL BIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Alkaline Phosphatase/metabolism | - |
dc.subject.MESH | Alveolar Process/cytology* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis/drug effects | - |
dc.subject.MESH | Calcium Phosphates/pharmacology | - |
dc.subject.MESH | Catechin/analogs & derivatives* | - |
dc.subject.MESH | Catechin/pharmacology | - |
dc.subject.MESH | Cell Movement/drug effects | - |
dc.subject.MESH | Cell Proliferation/drug effects | - |
dc.subject.MESH | Child | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Flow Cytometry | - |
dc.subject.MESH | Gene Expression | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | In Vitro Techniques | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Osteogenesis/drug effects* | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.title | The effect of epigallocatechin-3-gallate (EGCG) on human alveolar bone cells both in vitro and in vivo | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Pediatric Dentistry (소아치과학) | - |
dc.contributor.googleauthor | Yon-Joo Mah | - |
dc.contributor.googleauthor | Je Seon Song | - |
dc.contributor.googleauthor | Seong-Oh Kim | - |
dc.contributor.googleauthor | Jae-Ho Lee | - |
dc.contributor.googleauthor | Mijeong Jeon | - |
dc.contributor.googleauthor | Ui-Won Jung | - |
dc.contributor.googleauthor | Seok Jun Moon | - |
dc.contributor.googleauthor | Jeong-Hee Kim | - |
dc.contributor.googleauthor | Hyung-Jun Choi | - |
dc.identifier.doi | 10.1016/j.archoralbio.2014.02.011 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02058 | - |
dc.contributor.localId | A03134 | - |
dc.contributor.localId | A03511 | - |
dc.contributor.localId | A03692 | - |
dc.contributor.localId | A04216 | - |
dc.contributor.localId | A00577 | - |
dc.contributor.localId | A01358 | - |
dc.relation.journalcode | J00225 | - |
dc.identifier.eissn | 1879-1506 | - |
dc.identifier.pmid | 24637009 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0003996914000405 | - |
dc.subject.keyword | Alveolar bone-derived cells | - |
dc.subject.keyword | Epigallocatechin-3-gallate (EGCG) | - |
dc.subject.keyword | In vivo transplantation | - |
dc.subject.keyword | Osteogenic differentiation | - |
dc.contributor.alternativeName | Song, Je Seon | - |
dc.contributor.alternativeName | Lee, Jae Ho | - |
dc.contributor.alternativeName | Jeon, Mi Jeong | - |
dc.contributor.alternativeName | Jung, Ui Won | - |
dc.contributor.alternativeName | Choi, Hyung Jun | - |
dc.contributor.alternativeName | Kim, Seong Oh | - |
dc.contributor.alternativeName | Moon, Seok Jun | - |
dc.contributor.affiliatedAuthor | Song, Je Seon | - |
dc.contributor.affiliatedAuthor | Lee, Jae Ho | - |
dc.contributor.affiliatedAuthor | Jeon, Mi Jeong | - |
dc.contributor.affiliatedAuthor | Jung, Ui Won | - |
dc.contributor.affiliatedAuthor | Choi, Hyung Jun | - |
dc.contributor.affiliatedAuthor | Kim, Seong Oh | - |
dc.contributor.affiliatedAuthor | Moon, Seok Jun | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 59 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 539 | - |
dc.citation.endPage | 549 | - |
dc.identifier.bibliographicCitation | ARCHIVES OF ORAL BIOLOGY, Vol.59(5) : 539-549, 2014 | - |
dc.identifier.rimsid | 38144 | - |
dc.type.rims | ART | - |
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