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Localized surface plasmon resonance based nanobiosensor for biomarker detection of invasive cancer cells

Authors
 Yoochan Hong  ;  Minhee Ku  ;  Eugene Lee  ;  Jin-Suck Suh  ;  Yong-Min Huh  ;  Dae Sung Yoon  ;  Jaemoon Yang 
Citation
 JOURNAL OF BIOMEDICAL OPTICS, Vol.19(5) : 51202, 2014 
Journal Title
 JOURNAL OF BIOMEDICAL OPTICS 
ISSN
 1083-3668 
Issue Date
2014
MeSH
Biomarkers, Tumor/analysis* ; Biosensing Techniques/methods* ; Cell Line, Tumor ; Gold/chemistry ; Humans ; Immobilized Proteins/chemistry ; Immobilized Proteins/metabolism ; Matrix Metalloproteinase 14/chemistry ; Matrix Metalloproteinase 14/metabolism ; Nanotubes/chemistry ; Neoplasm Invasiveness ; Proteolysis ; Surface Plasmon Resonance/methods*
Keywords
Cancer ; Dielectrics ; Nanoparticles ; Surface plasmons ; Sensors ; Signal detection ; Glasses ; Refractive index ; Gold ; Metals
Abstract
In this study, we describe the development of a cancer biomarker-sensitive nanobiosensor based on localized surface plasmon resonance that enables recognition for proteolytic activity of membrane type 1 matrix metalloproteinase (MT1-MMP) anchored on invasive cancer cells. First of all, we prepared biomarker-detectable substrate based on gold nanorods (GNRs) using nanoparticle adsorption method. The sensitivity of the sensing chip was confirmed using various solvents that have different refractive indexes. Subsequently, MT1-MMP-specific cleavable peptide was conjugated onto the surface of GNRs, and molecular sensing about proteolytic activity was conducted using MT1-MMP and cell lysates. Collectively, we developed a biomarker detectable sensor, which allows for the effective detection of proteolytic activity about MT1-MMP extracted from invasive cancer cells.
Full Text
http://biomedicaloptics.spiedigitallibrary.org/article.aspx?articleid=1787519
DOI
10.1117/1.JBO.19.5.051202
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Ku, Min Hee(구민희) ORCID logo https://orcid.org/0000-0002-1674-1474
Suh, Jin Suck(서진석) ORCID logo https://orcid.org/0000-0001-9455-9240
Yang, Jae Moon(양재문) ORCID logo https://orcid.org/0000-0001-7365-0395
Lee, Eu Gene(이유진)
Huh, Yong Min(허용민) ORCID logo https://orcid.org/0000-0002-9831-4475
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/98035
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