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Clinical and anatomical approach using Sihler's staining technique (whole mount nerve stain).

 Sung-Yoon Won  ;  Da-Hye Kim  ;  Hun-Mu Yang  ;  Jong-Tae Park  ;  Hyun-Ho Kwak  ;  Kyung-Seok Hu  ;  Hee-Jin Kim 
 Anatomy and Cell Biology, Vol.44(1) : 1-7, 2011 
Journal Title
 Anatomy and Cell Biology 
Issue Date
Artery ; Nerve ; Review ; Sihler's staining ; Whole mount nerve staining
Sihler's staining allows visualization of the nerve distribution within soft tissues without extensive dissection and does not require slide preparation, unlike traditional approaches. This technique can be applied to the mucosa, muscle, and organs that contain myelinated nerve fibers. In particular, Sihler's technique may be considered the best tool for observing nerve distribution within skeletal muscles. The intramuscular distribution pattern of nerves is difficult to observe through manual manipulation due to the gradual tapering of nerves toward the terminal end of muscles, so it should be accompanied by histological studies to establish the finer branches therein. This method provides useful information not only for anatomists but also for physiologists and clinicians. Advanced knowledge of the nerve distribution patterns will be useful for developing guidelines for clinicians who perform operations such as muscle resection, tendon transplantation, and botulinum toxin injection. Furthermore, it is a useful technique to develop neurosurgical techniques and perform electrophysiological experiments. In this review, Sihler's staining technique is described in detail, covering its history, staining protocol, advantages, disadvantages, and possible applications. The application of this technique for determining the arterial distribution pattern is also described additionally in this study.
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Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Human Identification Research Center (개인식별연구소) > 1. Journal Papers
Yonsei Authors
Kim, Da Hye(김다혜)
Kim, Hee Jin(김희진) ORCID logo https://orcid.org/0000-0002-1139-6261
Park, Jong Tae(박종태)
Yang, Hun Mu(양헌무) ORCID logo https://orcid.org/0000-0003-1954-0114
Won, Sung Yoon(원성윤)
Hu, Kyung Seok(허경석) ORCID logo https://orcid.org/0000-0002-9048-3805
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