3 167

Cited 21 times in

Protease-activated receptor 2-dependent fluid secretion from airway submucosal glands by house dust mite extract

Authors
 Hyung-Ju Cho  ;  Hyun Jae Lee  ;  Sang Cheol Kim  ;  Kyubo Kim  ;  Yoo Suk Kim  ;  Chang-Hoon Kim  ;  Jeung-Gweon Lee  ;  Joo-Heon Yoon  ;  Jae Young Choi 
Citation
 Journal of Allergy and Clinical Immunology, Vol.129(2) : 529-535, 2012 
Journal Title
 Journal of Allergy and Clinical Immunology 
ISSN
 0091-6749 
Issue Date
2012
MeSH
Adolescent ; Adult ; Aged ; Antigens, Dermatophagoides/immunology* ; Carbachol/pharmacology ; Exocrine Glands/secretion* ; Female ; Humans ; Immunoglobulin E/blood ; Immunoglobulin E/immunology ; Male ; Middle Aged ; Mucus/secretion ; Nasal Mucosa/secretion* ; Oligopeptides/pharmacology ; Piperazines/pharmacology ; Receptor, PAR-2/agonists ; Receptor, PAR-2/antagonists & inhibitors ; Receptor, PAR-2/immunology* ; Rhinitis, Allergic, Perennial/immunology* ; Sinusitis/immunology* ; Young Adult
Keywords
House dust mites ; submucosal gland ; allergy ; chronic rhinosinusitis ; protease-activated receptor ; nasal
Abstract
BACKGROUND: The submucosal gland (SMG) is important in the control of airway surface fluid. Protease-activated receptor (PAR) 2 contributes to the pathophysiology of allergies in response to nonspecific allergens bearing proteases and anion secretion. House dust mites (HDMs) have abundant proteases that can activate PAR2, but little is known about the direct effect of HDM on SMG secretion. OBJECTIVE: The aim of this study was to investigate the effect of HDMs on glandular secretion and its mechanism in allergic patients, patients with chronic rhinosinusitis (CRS), or both. METHODS: Inferior nasal turbinates were harvested from 55 patients and classified into 4 groups (the control, allergic rhinitis [AR], CRS, and AR+CRS groups). A microscope attached to a digital camera was used to quantify mucus bubbles from individual SMGs while stimulated with HDM extract, PAR2-activating peptide, and carbachol. PAR2 expression in the SMG was determined by means of immunostaining with anti-PAR2 mAb. RESULTS: HDM induced a significantly higher secretion rate and number of responding glands in the AR and AR+CRS groups than in the control group. Interestingly, patients in the CRS group, who had no HDM-specific IgE antibody, showed a higher response than the control group, and its response was suppressed by a PAR2-selective antagonist. The responses to PAR2-activating peptide were similar to those to HDM, and their secretion rates positively correlated with HDM responses. PAR2 was highly expressed in all 3 disease groups with immunostaining. CONCLUSIONS: HDM allergens can induce glandular secretion in patients with AR, CRS, or both, and PAR2 represents a possible mechanism for nonspecific hyperreactivity in inflammatory airway diseases.
Full Text
http://www.sciencedirect.com/science/article/pii/S009167491101829X
DOI
22196772
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyu Bo(김규보)
Kim, Sang Cheol(김상철)
Kim, Yoo Suk(김유석)
Kim, Chang Hoon(김창훈) ORCID logo https://orcid.org/0000-0003-1238-6396
Yoon, Joo Heon(윤주헌)
Lee, Jeung Gweon(이정권)
Lee, Hyun Jae(이현재)
Cho, Hyung Ju(조형주) ORCID logo https://orcid.org/0000-0002-2851-3225
Choi, Jae Young(최재영) ORCID logo https://orcid.org/0000-0001-9493-3458
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/90609
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse