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Protease-activated receptor 2-dependent fluid secretion from airway submucosal glands by house dust mite extract

Authors
 Hyung-Ju Cho ; Hyun Jae Lee ; Jae Young Choi ; Joo-Heon Yoon ; Jeung-Gweon Lee ; Chang-Hoon Kim ; Yoo Suk Kim ; Kyubo Kim ; Sang Cheol Kim 
Citation
 Journal of Allergy and Clinical Immunology, Vol.129(2) : 529~535, 2012 
Journal Title
 Journal of Allergy and Clinical Immunology 
ISSN
 0091-6749 
Issue Date
2012
Abstract
BACKGROUND: The submucosal gland (SMG) is important in the control of airway surface fluid. Protease-activated receptor (PAR) 2 contributes to the pathophysiology of allergies in response to nonspecific allergens bearing proteases and anion secretion. House dust mites (HDMs) have abundant proteases that can activate PAR2, but little is known about the direct effect of HDM on SMG secretion. OBJECTIVE: The aim of this study was to investigate the effect of HDMs on glandular secretion and its mechanism in allergic patients, patients with chronic rhinosinusitis (CRS), or both. METHODS: Inferior nasal turbinates were harvested from 55 patients and classified into 4 groups (the control, allergic rhinitis [AR], CRS, and AR+CRS groups). A microscope attached to a digital camera was used to quantify mucus bubbles from individual SMGs while stimulated with HDM extract, PAR2-activating peptide, and carbachol. PAR2 expression in the SMG was determined by means of immunostaining with anti-PAR2 mAb. RESULTS: HDM induced a significantly higher secretion rate and number of responding glands in the AR and AR+CRS groups than in the control group. Interestingly, patients in the CRS group, who had no HDM-specific IgE antibody, showed a higher response than the control group, and its response was suppressed by a PAR2-selective antagonist. The responses to PAR2-activating peptide were similar to those to HDM, and their secretion rates positively correlated with HDM responses. PAR2 was highly expressed in all 3 disease groups with immunostaining. CONCLUSIONS: HDM allergens can induce glandular secretion in patients with AR, CRS, or both, and PAR2 represents a possible mechanism for nonspecific hyperreactivity in inflammatory airway diseases.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/90609
DOI
10.1016/j.jaci.2011.11.024
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Yonsei Biomedical Research Center
1. 연구논문 > 1. College of Medicine > Dept. of Otorhinolaryngology
Yonsei Authors
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Link
 http://www.sciencedirect.com/science/article/pii/S009167491101829X
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