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Comparative Gene Expression Analysis of the Human Periodontal Ligament in Deciduous and Permanent Teeth

Authors
 Je Seon Song  ;  Dong Hwan Hwang  ;  Seong-Oh Kim  ;  Mijeong Jeon  ;  Byung-Jai Choi  ;  Han-Sung Jung  ;  Seok Jun Moon  ;  Wonse Park  ;  Hyung-Jun Choi 
Citation
 PLoS One, Vol.8(4) : e61231, 2013 
Journal Title
 PLoS One 
ISSN
 1932-6203 
Issue Date
2013
Abstract
There are histological and functional differences between human deciduous and permanent periodontal ligament (PDL) tissues. The aim of this study was to determine the differences between these two types of tissue at the molecular level by comparing their gene expression patterns. PDL samples were obtained from permanent premolars (n = 38) and anterior deciduous teeth (n = 31) extracted from 40 healthy persons. Comparative cDNA microarray analysis revealed several differences in gene expression between the deciduous and permanent PDL tissues. These findings were verified by qRT-PCR (quantitative reverse-transcription–polymerase chain reaction) analysis, and the areas where genes are expressed were revealed by immunohistochemical staining. The expressions of 21 genes were up-regulated in deciduous relative to PDL tissues, and those of 30 genes were up-regulated in permanent relative to deciduous PDL tissues. The genes that were up-regulated in deciduous PDL tissues were those involved in the formation of the extracellular matrix (LAMC2, LAMB3, and COMP), tissue development (IGF2BP, MAB21L2, and PAX3), and inflammatory or immune reactions leading to tissue degradation (IL1A, CCL21, and CCL18). The up-regulated genes in permanent PDL tissues were related to tissue degradation (IL6 and ADAMTS18), myocontraction (PDE3B, CASQ2, and MYH10), and neurological responses (FOS, NCAM2, SYT1, SLC22A3, DOCK3, LRRTM1, LRRTM3, PRSS12, and ARPP21). The analysis of differential gene expressions between deciduous and permanent PDL tissues aids our understanding of histological and functional differences between them at the molecular level.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/86608
Files in This Item:
T201300755.pdf Download
DOI
10.1371/journal.pone.0061231
Appears in Collections:
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Advanced General Dentistry (통합치의학과)
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실)
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실)
1. Journal Papers (연구논문) > 5. Research Institutes (연구소) > Oral Science Research Center (구강과학연구소)
Yonsei Authors
김성오(Kim, Seong Oh) ORCID logo https://orcid.org/0000-0002-8620-1377
문석준(Moon, Seok Jun)
박원서(Park, Wonse)
송제선(Song, Je Seon) ORCID logo https://orcid.org/0000-0001-8620-5629
전미정(Jeon, Mi Jeong)
정한성(Jung, Han Sung)
최병재(Choi, Byung Jai)
최형준(Choi, Hyung Jun)
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