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Isolation of glioma cancer stem cells in relation to histological grades in glioma specimens.

 Byung Ho Kong  ;  Na-Ri Park  ;  Jin-Kyoung Shim  ;  Bo-Kyung Kim  ;  Hye-Jin Shin  ;  Ji-Hyun Lee  ;  Yong-Min Huh  ;  Su-Jae Lee  ;  Se-Hoon Kim  ;  Eui-Hyun Kim  ;  Eun-Kyung Park  ;  Jong Hee Chang  ;  Dong-Seok Kim  ;  Sun Ho Kim  ;  Yong-Kil Hong  ;  Seok-Gu Kang  ;  Frederick F. Lang 
 CHILDS NERVOUS SYSTEM, Vol.29(2) : 217-229, 2013 
Journal Title
Issue Date
Adolescent ; Adult ; Aged ; Animals ; Brain Neoplasms/pathology* ; Cell Differentiation/physiology ; Cell Separation ; Child, Preschool ; Female ; Glioma/pathology* ; Humans ; Male ; Mice ; Mice, Nude ; Middle Aged ; Neoplasm Grading ; Neoplastic Stem Cells/pathology* ; Xenograft Model Antitumor Assays/methods
Glioma ; Glioma cancer stem cell ; Gliomasphere ; Histological grade ; Isolation
PURPOSE: The existence of cancer stem cells (CSCs) in glioblastoma has been proposed. However, the unknown knowledge that is yet to be revealed is the presence of glioma CSCs (gCSCs) in correlation to each WHO grades of glioma. We approached this study with a hypothesis that specimens from high-grade gliomas would have higher isolation rate of gCSCs in comparison to those of lower-grade gliomas. METHODS: The glioma specimens were obtained from patients and underwent gliomasphere assay. The gliomaspheres were chosen to be analyzed with immunocytochemisty for surface markers. Then the selected gliomaspheres were exposed to neural differentiation conditions. Lastly, we made mouse orthotopic glioma models to examine the capacity of gliomagenesis. RESULTS: The gliomaspheres were formed in WHO grade IV (13 of 21) and III (two of nine) gliomas. Among them, WHO grade IV (11 of 13) and III (two of two) gliomaspheres showed similar surface markers to gCSCs and were capable of neural differentiation. Lastly, among the chosen cells, 10 of 11 WHO grade IV and two of two WHO grade III gliomaspheres were capable of gliomagenesis. Thus, overall, the rates of existence of gCSCs were more prominent in high-grade gliomas: 47.6% (10 of 21) in WHO grade IV gliomas and 22.2% (two of nine) in WHO grade III gliomas, whereas WHO grade II and I gliomas showed virtually no gCSCs. CONCLUSIONS: This trend of stage-by-stage increase of gCSCs in gliomas showed statistical significance by chi-square test linear-by-linear association. We prove that the rates of existence of gCSCs increase proportionally as the WHO grades of gliomas rise.
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1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kang, Seok Gu(강석구) ORCID logo https://orcid.org/0000-0001-5676-2037
Kim, Dong Seok(김동석)
Kim, Bo Kyung(김보경)
Kim, Sun Ho(김선호) ORCID logo https://orcid.org/0000-0003-0970-3848
Kim, Se Hoon(김세훈) ORCID logo https://orcid.org/0000-0001-7516-7372
Kim, Eui Hyun(김의현) ORCID logo https://orcid.org/0000-0002-2523-7122
Park, Eun Kyung(박은경)
Lee, Ji Hyun(이지현) ORCID logo https://orcid.org/0000-0002-9223-9478
Chang, Jong Hee(장종희)
Huh, Yong Min(허용민) ORCID logo https://orcid.org/0000-0002-9831-4475
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