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Isolation of glioma cancer stem cells in relation to histological grades in glioma specimens.

DC Field Value Language
dc.contributor.author김선호-
dc.contributor.author김세훈-
dc.contributor.author김의현-
dc.contributor.author박은경-
dc.contributor.author이지현-
dc.contributor.author강석구-
dc.contributor.author장종희-
dc.contributor.author김동석-
dc.contributor.author허용민-
dc.contributor.author김보경-
dc.date.accessioned2014-12-18T08:20:24Z-
dc.date.available2014-12-18T08:20:24Z-
dc.date.issued2013-
dc.identifier.issn0256-7040-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/86124-
dc.description.abstractPURPOSE: The existence of cancer stem cells (CSCs) in glioblastoma has been proposed. However, the unknown knowledge that is yet to be revealed is the presence of glioma CSCs (gCSCs) in correlation to each WHO grades of glioma. We approached this study with a hypothesis that specimens from high-grade gliomas would have higher isolation rate of gCSCs in comparison to those of lower-grade gliomas. METHODS: The glioma specimens were obtained from patients and underwent gliomasphere assay. The gliomaspheres were chosen to be analyzed with immunocytochemisty for surface markers. Then the selected gliomaspheres were exposed to neural differentiation conditions. Lastly, we made mouse orthotopic glioma models to examine the capacity of gliomagenesis. RESULTS: The gliomaspheres were formed in WHO grade IV (13 of 21) and III (two of nine) gliomas. Among them, WHO grade IV (11 of 13) and III (two of two) gliomaspheres showed similar surface markers to gCSCs and were capable of neural differentiation. Lastly, among the chosen cells, 10 of 11 WHO grade IV and two of two WHO grade III gliomaspheres were capable of gliomagenesis. Thus, overall, the rates of existence of gCSCs were more prominent in high-grade gliomas: 47.6% (10 of 21) in WHO grade IV gliomas and 22.2% (two of nine) in WHO grade III gliomas, whereas WHO grade II and I gliomas showed virtually no gCSCs. CONCLUSIONS: This trend of stage-by-stage increase of gCSCs in gliomas showed statistical significance by chi-square test linear-by-linear association. We prove that the rates of existence of gCSCs increase proportionally as the WHO grades of gliomas rise.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfCHILDS NERVOUS SYSTEM-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdolescent-
dc.subject.MESHAdult-
dc.subject.MESHAged-
dc.subject.MESHAnimals-
dc.subject.MESHBrain Neoplasms/pathology*-
dc.subject.MESHCell Differentiation/physiology-
dc.subject.MESHCell Separation-
dc.subject.MESHChild, Preschool-
dc.subject.MESHFemale-
dc.subject.MESHGlioma/pathology*-
dc.subject.MESHHumans-
dc.subject.MESHMale-
dc.subject.MESHMice-
dc.subject.MESHMice, Nude-
dc.subject.MESHMiddle Aged-
dc.subject.MESHNeoplasm Grading-
dc.subject.MESHNeoplastic Stem Cells/pathology*-
dc.subject.MESHXenograft Model Antitumor Assays/methods-
dc.titleIsolation of glioma cancer stem cells in relation to histological grades in glioma specimens.-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentYonsei Biomedical Research Center (연세의생명연구원)-
dc.contributor.googleauthorByung Ho Kong-
dc.contributor.googleauthorNa-Ri Park-
dc.contributor.googleauthorJin-Kyoung Shim-
dc.contributor.googleauthorBo-Kyung Kim-
dc.contributor.googleauthorHye-Jin Shin-
dc.contributor.googleauthorJi-Hyun Lee-
dc.contributor.googleauthorYong-Min Huh-
dc.contributor.googleauthorSu-Jae Lee-
dc.contributor.googleauthorSe-Hoon Kim-
dc.contributor.googleauthorEui-Hyun Kim-
dc.contributor.googleauthorEun-Kyung Park-
dc.contributor.googleauthorJong Hee Chang-
dc.contributor.googleauthorDong-Seok Kim-
dc.contributor.googleauthorSun Ho Kim-
dc.contributor.googleauthorYong-Kil Hong-
dc.contributor.googleauthorSeok-Gu Kang-
dc.contributor.googleauthorFrederick F. Lang-
dc.identifier.doi10.1007/s00381-012-1964-9-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00560-
dc.contributor.localIdA00610-
dc.contributor.localIdA00837-
dc.contributor.localIdA01607-
dc.contributor.localIdA00036-
dc.contributor.localIdA03470-
dc.contributor.localIdA00402-
dc.contributor.localIdA04359-
dc.contributor.localIdA03218-
dc.contributor.localIdA00506-
dc.relation.journalcodeJ00525-
dc.identifier.eissn1433-0350-
dc.identifier.pmid23143002-
dc.identifier.urlhttp://link.springer.com/article/10.1007%2Fs00381-012-1964-9-
dc.subject.keywordGlioma-
dc.subject.keywordGlioma cancer stem cell-
dc.subject.keywordGliomasphere-
dc.subject.keywordHistological grade-
dc.subject.keywordIsolation-
dc.contributor.alternativeNameKim, Sun Ho-
dc.contributor.alternativeNameKim, Se Hoon-
dc.contributor.alternativeNameKim, Eui Hyun-
dc.contributor.alternativeNamePark, Eun Kyung-
dc.contributor.alternativeNameLee, Ji Hyun-
dc.contributor.alternativeNameKang, Seok Gu-
dc.contributor.alternativeNameChang, Jong Hee-
dc.contributor.alternativeNameKim, Dong Seok-
dc.contributor.alternativeNameHuh, Yong Min-
dc.contributor.alternativeNameKim, Bo Kyung-
dc.contributor.affiliatedAuthorKim, Sun Ho-
dc.contributor.affiliatedAuthorKim, Se Hoon-
dc.contributor.affiliatedAuthorKim, Eui Hyun-
dc.contributor.affiliatedAuthorPark, Eun Kyung-
dc.contributor.affiliatedAuthorKang, Seok Gu-
dc.contributor.affiliatedAuthorChang, Jong Hee-
dc.contributor.affiliatedAuthorKim, Dong Seok-
dc.contributor.affiliatedAuthorHuh, Yong Min-
dc.contributor.affiliatedAuthorLee, Ji Hyun-
dc.contributor.affiliatedAuthorKim, Bo Kyung-
dc.rights.accessRightsnot free-
dc.citation.volume29-
dc.citation.number2-
dc.citation.startPage217-
dc.citation.endPage229-
dc.identifier.bibliographicCitationCHILDS NERVOUS SYSTEM, Vol.29(2) : 217-229, 2013-
dc.identifier.rimsid28798-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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