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In Vivo Evaluation of Decellularized Human Tooth Scaffold for Dental Tissue Regeneration

 Ik-Hwan Kim  ;  Mijeong Jeon  ;  Kyounga Cheon  ;  Sun Ha Kim  ;  Han-Sung Jung  ;  Yooseok Shin  ;  Chung Min Kang  ;  Seong-Oh Kim  ;  Hyung-Jun Choi  ;  Hyo-Seol Lee  ;  Ko Eun Lee  ;  Je Seon Song 
 APPLIED SCIENCES-BASEL, Vol.11(18) : 8472, 2021-11 
Journal Title
Issue Date
decellularized tooth scaffold ; dental pulp stem cell ; periodontal ligament stem cell ; tissue regeneration
Conventional root canal treatment may result in loss of tooth vitality, which can lead to unfavorable treatment outcomes. Notably, a ceased tooth development of immature permanent teeth with open apices, regeneration of periodontal ligaments (PDL), and pulp is highly expected healing process. For regeneration, the scaffold is one of the critical components that carry biological benefits. Therefore, this study evaluated a decellularized human tooth as a scaffold for the PDL and pulp tissue regeneration. A tooth scaffold was fabricated using an effective decellularization method as reported in previous studies. PDL stem cells (PDLSCs) and dental pulp stem cells (DPSCs) obtained from human permanent teeth were inoculated onto decellularized scaffolds, then cultured to transplant into immunosuppressed mouse. After 9 weeks, PDLSCs and DPSCs that were inoculated onto decellularized tooth scaffolds and cultured in an in vivo demonstrated successful differentiation. In PDLSCs, a regeneration of the cementum/PDL complex could be expected. In DPSCs, the expression of genes related to revascularization and the hard tissue regeneration showed the possibility of pulp regeneration. This study suggested that the potential possible application of decellularized human tooth could be a scaffold in regeneration PDL and pulp tissue along with PDLSCs and DPSCs, respectively, as a novel treatment method.
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2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Chung Min(강정민) ORCID logo https://orcid.org/0000-0001-7813-3741
Kim, Seong Oh(김성오) ORCID logo https://orcid.org/0000-0002-8620-1377
Kim, Ik-Hwan(김익환)
Song, Je Seon(송제선) ORCID logo https://orcid.org/0000-0001-8620-5629
Shin, Yoo Seok(신유석) ORCID logo https://orcid.org/0000-0003-1121-2570
Jeon, Mi Jeong(전미정)
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
Choi, Hyung Jun(최형준) ORCID logo https://orcid.org/0000-0002-3315-6912
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