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The Effect of Mechanical Vibration on Osteogenesis of Periodontal Ligament Stem Cells

Authors
 Dongzi Chen  ;  Sunil Kim  ;  Sukjoon Lee  ;  Jong-Min Lee  ;  Yoon Jeong Choi  ;  Su-Jung Shin  ;  Han-Sung Jung  ;  Euiseong Kim 
Citation
 JOURNAL OF ENDODONTICS, Vol.47(11) : 1767-1774, 2021-11 
Journal Title
JOURNAL OF ENDODONTICS
ISSN
 0099-2399 
Issue Date
2021-11
Keywords
Ankylosis ; bite force ; periodontium ; regeneration ; tooth replantation
Abstract
Introduction: Appropriate occlusal forces can prevent ankylosis after tooth replantation or transplantation. However, the "proper occlusal forces" on periodontal ligament (PDL) healing have not yet been defined due to insufficient in vitro studies and uncertain in vitro models. Herein, we presented a mechanical vibration device as an in vitro model to determine such favorable occlusal forces.

Methods: Human periodontal ligament stem cells (hPDLSCs) were exposed to mechanical vibration force with 4 frequencies (30, 90, 150, and 210 rpm). Cell viability and the expression of osteogenic differentiation-related genes and proteins were tested in vitro. The calvarial transplantation experiment was performed to assess the bone formation ability of 150 rpm mechanical vibration stimulation (MVS).

Results: MVS at 150 and 210 rpm significantly reduced cell viability in the early stages. The 150-rpm MVS decreased osteogenic marker expression at the early time point (3 days) but had no harmful effects at the late time point (14 days). Furthermore, hPDLSC cell sheets treated with 150-rpm MVS had potential to decrease bone formation in rat calvarial defects serendipitously and facilitated functional PDL-like tissue formation.

Conclusions: We found that MVS at a frequency of 150 rpm could provide a strategy for a transient reduction in the osteogenic potential of hPDLSCs and promote PDL-like tissue formation. Thus, 150-rpm MVS could be used as a controllable proper occlusal force to prevent ankylosis and promote PDL healing after tooth replantation or transplantation.
Full Text
https://www.sciencedirect.com/science/article/pii/S0099239921006373
DOI
10.1016/j.joen.2021.08.014
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Orthodontics (교정과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Others (기타) > 1. Journal Papers
Yonsei Authors
Kim, Sun Il(김선일) ORCID logo https://orcid.org/0000-0002-8889-9844
Kim, Eui Seong(김의성) ORCID logo https://orcid.org/0000-0003-2126-4761
Shin, Su Jung(신수정) ORCID logo https://orcid.org/0000-0001-5795-3919
Lee, Sukjoon(이석준)
Lee, Jong Min(이종민) ORCID logo https://orcid.org/0000-0002-9466-7644
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
Choi, Yoon Jeong(최윤정) ORCID logo https://orcid.org/0000-0003-0781-8836
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/187211
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