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Intranuclear Delivery of Nuclear Factor-Kappa B p65 in a Rat Model of Tooth Replantation

Authors
 Chung-Min Kang  ;  Seunghan Mo  ;  Mijeong Jeon  ;  Ui-Won Jung  ;  Yooseok Shin  ;  Jin-Su Shin  ;  Bo-Young Shin  ;  Sang-Kyou Lee  ;  Hyung-Jun Choi  ;  Je Seon Song 
Citation
 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.22(4) : 1987, 2021-02 
Journal Title
 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 
Issue Date
2021-02
MeSH
Animals ; Cell Differentiation ; Cell Nucleus / metabolism* ; Cell Survival ; Mice ; Models, Animal ; Molar / diagnostic imaging ; Nitric Oxide / metabolism ; Osteoclasts / metabolism ; RAW 264.7 Cells ; Rats ; Tooth Replantation* ; Transcription Factor RelA / metabolism* ; Transduction, Genetic ; X-Ray Microtomography
Keywords
intra-nucleus delivery ; nuclear factor kappa B ; osteoclast ; tooth replantation
Abstract
After avulsion and replantation, teeth are at risk of bone and root resorption. The present study aimed to demonstrate that the intra-nuclear transducible form of transcription modulation domain of p65 (nt-p65-TMD) can suppress osteoclast differentiation in vitro, and reduce bone resorption in a rat model of tooth replantation. Cell viability and nitric oxide release were evaluated in RAW264.7 cells using CCK-8 assay and Griess reaction kit. Osteoclast differentiation was evaluated using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and tartrate-resistant acid phosphatase (TRAP) staining. Thirty-two maxillary rat molars were extracted and stored in saline (n = 10) or 10 µM nt-p65-TMD solution (n = 22) before replantation. After 4 weeks, specimens were scored according to the inflammatory pattern using micro-computed tomography (CT) imaging and histological analyses. nt-p65-TMD treatment resulted in significant reduction of nitric oxide release and osteoclast differentiation as studied using PCR and TRAP staining. Further, micro-CT analysis revealed a significant decrease in bone resorption in the nt-p65-TMD treatment group (p < 0.05). Histological analysis of nt-p65-TMD treatment group showed that not only bone and root resorption, but also inflammation of the periodontal ligament and epithelial insertion was significantly reduced. These findings suggest that nt-p65-TMD has the unique capabilities of regulating bone remodeling after tooth replantation.
DOI
10.3390/ijms22041987
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Chung Min(강정민) ORCID logo https://orcid.org/0000-0001-7813-3741
Song, Je Seon(송제선) ORCID logo https://orcid.org/0000-0001-8620-5629
Shin, Yoo Seok(신유석) ORCID logo https://orcid.org/0000-0003-1121-2570
Jung, Ui Won(정의원) ORCID logo https://orcid.org/0000-0001-6371-4172
Choi, Hyung Jun(최형준) ORCID logo https://orcid.org/0000-0002-3315-6912
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/182165
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