Cited 2 times in
Intranuclear Delivery of Nuclear Factor-Kappa B p65 in a Rat Model of Tooth Replantation
DC Field | Value | Language |
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dc.contributor.author | 강정민 | - |
dc.contributor.author | 송제선 | - |
dc.contributor.author | 신유석 | - |
dc.contributor.author | 정의원 | - |
dc.contributor.author | 최형준 | - |
dc.date.accessioned | 2021-04-29T17:04:06Z | - |
dc.date.available | 2021-04-29T17:04:06Z | - |
dc.date.issued | 2021-02 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/182165 | - |
dc.description.abstract | After avulsion and replantation, teeth are at risk of bone and root resorption. The present study aimed to demonstrate that the intra-nuclear transducible form of transcription modulation domain of p65 (nt-p65-TMD) can suppress osteoclast differentiation in vitro, and reduce bone resorption in a rat model of tooth replantation. Cell viability and nitric oxide release were evaluated in RAW264.7 cells using CCK-8 assay and Griess reaction kit. Osteoclast differentiation was evaluated using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and tartrate-resistant acid phosphatase (TRAP) staining. Thirty-two maxillary rat molars were extracted and stored in saline (n = 10) or 10 µM nt-p65-TMD solution (n = 22) before replantation. After 4 weeks, specimens were scored according to the inflammatory pattern using micro-computed tomography (CT) imaging and histological analyses. nt-p65-TMD treatment resulted in significant reduction of nitric oxide release and osteoclast differentiation as studied using PCR and TRAP staining. Further, micro-CT analysis revealed a significant decrease in bone resorption in the nt-p65-TMD treatment group (p < 0.05). Histological analysis of nt-p65-TMD treatment group showed that not only bone and root resorption, but also inflammation of the periodontal ligament and epithelial insertion was significantly reduced. These findings suggest that nt-p65-TMD has the unique capabilities of regulating bone remodeling after tooth replantation. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.publisher | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Differentiation | - |
dc.subject.MESH | Cell Nucleus / metabolism* | - |
dc.subject.MESH | Cell Survival | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Models, Animal | - |
dc.subject.MESH | Molar / diagnostic imaging | - |
dc.subject.MESH | Nitric Oxide / metabolism | - |
dc.subject.MESH | Osteoclasts / metabolism | - |
dc.subject.MESH | RAW 264.7 Cells | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Tooth Replantation* | - |
dc.subject.MESH | Transcription Factor RelA / metabolism* | - |
dc.subject.MESH | Transduction, Genetic | - |
dc.subject.MESH | X-Ray Microtomography | - |
dc.title | Intranuclear Delivery of Nuclear Factor-Kappa B p65 in a Rat Model of Tooth Replantation | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Pediatric Dentistry (소아치과학교실) | - |
dc.contributor.googleauthor | Chung-Min Kang | - |
dc.contributor.googleauthor | Seunghan Mo | - |
dc.contributor.googleauthor | Mijeong Jeon | - |
dc.contributor.googleauthor | Ui-Won Jung | - |
dc.contributor.googleauthor | Yooseok Shin | - |
dc.contributor.googleauthor | Jin-Su Shin | - |
dc.contributor.googleauthor | Bo-Young Shin | - |
dc.contributor.googleauthor | Sang-Kyou Lee | - |
dc.contributor.googleauthor | Hyung-Jun Choi | - |
dc.contributor.googleauthor | Je Seon Song | - |
dc.identifier.doi | 10.3390/ijms22041987 | - |
dc.contributor.localId | A05078 | - |
dc.contributor.localId | A02058 | - |
dc.contributor.localId | A02129 | - |
dc.contributor.localId | A03692 | - |
dc.contributor.localId | A04216 | - |
dc.relation.journalcode | J01133 | - |
dc.identifier.eissn | 1422-0067 | - |
dc.identifier.pmid | 33671455 | - |
dc.subject.keyword | intra-nucleus delivery | - |
dc.subject.keyword | nuclear factor kappa B | - |
dc.subject.keyword | osteoclast | - |
dc.subject.keyword | tooth replantation | - |
dc.contributor.alternativeName | Kang, Chung Min | - |
dc.contributor.affiliatedAuthor | 강정민 | - |
dc.contributor.affiliatedAuthor | 송제선 | - |
dc.contributor.affiliatedAuthor | 신유석 | - |
dc.contributor.affiliatedAuthor | 정의원 | - |
dc.contributor.affiliatedAuthor | 최형준 | - |
dc.citation.volume | 22 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1987 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.22(4) : 1987, 2021-02 | - |
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