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피브린 글루를 이용한 인간골수유래줄기세포의 생체 내 연골형성

Other Titles
 In Vivo Cartilage Formation Using Human Bone Marrow-Derived Mesenchymal Stem Cells Mixed with Fibrin Glue 
 박기원  ;  최우진  ;  이경미  ;  윤동석  ;  김성환  ;  이진우 
 Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지), Vol.18(2) : 43-50, 2015 
Journal Title
 Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지) 
Issue Date
Purpose: To investigate the feasibility of in vivo cartilage formation by direct injection the chondrogenicundifferentiated human bone marrow-derived mesenchymal stem cells (hBMSCs) mixed with fibrin glue including TGF-β3. Materials and Methods: Chondrogenic differentiation induced hBMSCs for 14 days (control group-2) and undifferentiated hBMSCs combined with TGF-β3 mixed (experimental group-3) with fibrin glue and fibrin glue only (control group-1) were injected subcutanteously into the back of nude mouse. For evaluation of the cartilage-like tissue formed after 8 weeks after injection, real time PCR, histological analysis and immunohistochemical analysis were used. Results: Control group-1 did not form any visible mass. Control group-2 as well as experimental group-3 could form new cartilage-like tissue which were demonstrated expression of type II collagen by real-time PCR, histology analysis such as H&E staining, MT staining and type II collagen specific immunohistologic analysis. As results, expression of type II collagen was shown in the both groups. Conclusion: Our study confirmed that cartilage-like tissues could be formed in subcutaneous layer of nude mouse by direct injection mixed with fibrin glue including TGF-β3 without chondrogenic-induction of hBMSCs, suggesting that these model could be suitable for preliminary studies or optimizing experiments to evaluate reconstruction of cartilage.
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
김성환(Kim, Sung Hwan) ORCID logo https://orcid.org/0000-0001-5743-6241
박기원(Park, Ki Won)
윤동석(Yoon, Dong Suk)
이경미(Lee, Kyoung Mi) ORCID logo https://orcid.org/0000-0002-9038-8162
이진우(Lee, Jin Woo) ORCID logo https://orcid.org/0000-0002-0293-9017
최우진(Choi, Woo Jin)
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