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피브린 글루를 이용한 인간골수유래줄기세포의 생체 내 연골형성
DC Field | Value | Language |
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dc.contributor.author | 김성환 | - |
dc.contributor.author | 박기원 | - |
dc.contributor.author | 윤동석 | - |
dc.contributor.author | 이경미 | - |
dc.contributor.author | 이진우 | - |
dc.contributor.author | 최우진 | - |
dc.date.accessioned | 2018-03-26T16:59:15Z | - |
dc.date.available | 2018-03-26T16:59:15Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1226-8933 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/157047 | - |
dc.description.abstract | Purpose: To investigate the feasibility of in vivo cartilage formation by direct injection the chondrogenicundifferentiated human bone marrow-derived mesenchymal stem cells (hBMSCs) mixed with fibrin glue including TGF-β3. Materials and Methods: Chondrogenic differentiation induced hBMSCs for 14 days (control group-2) and undifferentiated hBMSCs combined with TGF-β3 mixed (experimental group-3) with fibrin glue and fibrin glue only (control group-1) were injected subcutanteously into the back of nude mouse. For evaluation of the cartilage-like tissue formed after 8 weeks after injection, real time PCR, histological analysis and immunohistochemical analysis were used. Results: Control group-1 did not form any visible mass. Control group-2 as well as experimental group-3 could form new cartilage-like tissue which were demonstrated expression of type II collagen by real-time PCR, histology analysis such as H&E staining, MT staining and type II collagen specific immunohistologic analysis. As results, expression of type II collagen was shown in the both groups. Conclusion: Our study confirmed that cartilage-like tissues could be formed in subcutaneous layer of nude mouse by direct injection mixed with fibrin glue including TGF-β3 without chondrogenic-induction of hBMSCs, suggesting that these model could be suitable for preliminary studies or optimizing experiments to evaluate reconstruction of cartilage. | - |
dc.description.statementOfResponsibility | prohibition | - |
dc.language | Korean | - |
dc.publisher | 대한정형외과연구학회 | - |
dc.relation.isPartOf | Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 피브린 글루를 이용한 인간골수유래줄기세포의 생체 내 연골형성 | - |
dc.title.alternative | In Vivo Cartilage Formation Using Human Bone Marrow-Derived Mesenchymal Stem Cells Mixed with Fibrin Glue | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Orthopedic Surgery | - |
dc.contributor.googleauthor | 박기원 | - |
dc.contributor.googleauthor | 최우진 | - |
dc.contributor.googleauthor | 이경미 | - |
dc.contributor.googleauthor | 윤동석 | - |
dc.contributor.googleauthor | 김성환 | - |
dc.contributor.googleauthor | 이진우 | - |
dc.contributor.localId | A00592 | - |
dc.contributor.localId | A05239 | - |
dc.contributor.localId | A02547 | - |
dc.contributor.localId | A04619 | - |
dc.contributor.localId | A03230 | - |
dc.contributor.localId | A04121 | - |
dc.relation.journalcode | J01526 | - |
dc.contributor.alternativeName | Kim, Sung Hwan | - |
dc.contributor.alternativeName | Park, Ki Won | - |
dc.contributor.alternativeName | Yoon, Dong Suk | - |
dc.contributor.alternativeName | Lee, Kyoung Mi | - |
dc.contributor.alternativeName | Lee, Jin Woo | - |
dc.contributor.alternativeName | Choi, Woo Jin | - |
dc.contributor.affiliatedAuthor | Kim, Sung Hwan | - |
dc.contributor.affiliatedAuthor | Park, Ki Won | - |
dc.contributor.affiliatedAuthor | Yoon, Dong Suk | - |
dc.contributor.affiliatedAuthor | Lee, Kyoung Mi | - |
dc.contributor.affiliatedAuthor | Lee, Jin Woo | - |
dc.contributor.affiliatedAuthor | Choi, Woo Jin | - |
dc.citation.volume | 18 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 43 | - |
dc.citation.endPage | 50 | - |
dc.identifier.bibliographicCitation | Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지), Vol.18(2) : 43-50, 2015 | - |
dc.identifier.rimsid | 41355 | - |
dc.type.rims | ART | - |
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