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Local myogenic pulp-derived cell injection enhances craniofacial muscle regeneration in vivo

 J. E. Jung  ;  M. J. Song  ;  S. Shin  ;  Y. J. Choi  ;  K. H. Kim  ;  C. J. Chung 
 Orthodontics & Craniofacial Research, Vol.20(1) : 35-43, 2017 
Journal Title
 Orthodontics & Craniofacial Research 
Issue Date
Adolescent ; Azacitidine/analogs & derivatives* ; Azacitidine/pharmacology ; Bicuspid ; Cell Differentiation*/drug effects ; Cells, Cultured ; DNA Methylation ; Dental Pulp/cytology* ; Humans ; Masseter Muscle/physiology* ; Muscle Development/physiology* ; Muscle, Skeletal/physiology ; Regeneration/drug effects ; Regeneration/physiology* ; Young Adult
5-aza-2′-deoxycytidine ; muscle injury ; myogenesis ; pulp cell ; regeneration
OBJECTIVES: To enhance myogenic differentiation in pulp cells isolated from extracted premolars by epigenetic modification using a DNA demethylation agent, 5-aza-2'-deoxycytidine (5-Aza), and to evaluate the potent stimulatory effect of 5-Aza-treated pulp cell injection for craniofacial muscle regeneration in vivo. SETTING AND SAMPLE POPULATION: Pulp cells were isolated from premolars extracted for orthodontic purposes from four adults (age range, 18-22.1 years). MATERIAL AND METHODS: Levels of myogenic differentiation and functional contraction response in vitro were compared between pulp cells with or without pre-treatment of 5-Aza. Changes in muscle regeneration in response to green fluorescent protein (GFP)-labelled myogenic pulp cell injection in vivo were evaluated using a cardiotoxin (CTX)-induced muscle injury model of the gastrocnemius as well as the masseter muscle in mice. RESULTS: Pre-treatment of 5-Aza in pulp cells stimulated myotube formation, myogenic differentiation in terms of desmin and myogenin expression, and the level of collagen gel contraction. The local injection of 5-Aza pre-treated myogenic pulp cells was engrafted into the host tissue and indicated signs of enhanced muscle regeneration in both the gastrocnemius and the masseter muscles. CONCLUSION: The epigenetic modification of pulp cells from extracted premolars and the local injection of myogenic pulp cells may stimulate craniofacial muscles regeneration in vivo.
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2. College of Dentistry (치과대학) > Dept. of Orthodontics (교정과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Ho(김경호) ORCID logo https://orcid.org/0000-0002-8154-2041
Shin, Su Jung(신수정) ORCID logo https://orcid.org/0000-0001-5795-3919
Chung, Chooryung J.(정주령) ORCID logo https://orcid.org/0000-0001-9399-7193
Choi, Yoon Jeong(최윤정) ORCID logo https://orcid.org/0000-0003-0781-8836
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