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Local myogenic pulp-derived cell injection enhances craniofacial muscle regeneration in vivo

DC Field Value Language
dc.contributor.author김경호-
dc.contributor.author최윤정-
dc.contributor.author신수정-
dc.contributor.author정주령-
dc.date.accessioned2017-11-02T08:05:41Z-
dc.date.available2017-11-02T08:05:41Z-
dc.date.issued2017-
dc.identifier.issn1601-6335-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/154068-
dc.description.abstractOBJECTIVES: To enhance myogenic differentiation in pulp cells isolated from extracted premolars by epigenetic modification using a DNA demethylation agent, 5-aza-2'-deoxycytidine (5-Aza), and to evaluate the potent stimulatory effect of 5-Aza-treated pulp cell injection for craniofacial muscle regeneration in vivo. SETTING AND SAMPLE POPULATION: Pulp cells were isolated from premolars extracted for orthodontic purposes from four adults (age range, 18-22.1 years). MATERIAL AND METHODS: Levels of myogenic differentiation and functional contraction response in vitro were compared between pulp cells with or without pre-treatment of 5-Aza. Changes in muscle regeneration in response to green fluorescent protein (GFP)-labelled myogenic pulp cell injection in vivo were evaluated using a cardiotoxin (CTX)-induced muscle injury model of the gastrocnemius as well as the masseter muscle in mice. RESULTS: Pre-treatment of 5-Aza in pulp cells stimulated myotube formation, myogenic differentiation in terms of desmin and myogenin expression, and the level of collagen gel contraction. The local injection of 5-Aza pre-treated myogenic pulp cells was engrafted into the host tissue and indicated signs of enhanced muscle regeneration in both the gastrocnemius and the masseter muscles. CONCLUSION: The epigenetic modification of pulp cells from extracted premolars and the local injection of myogenic pulp cells may stimulate craniofacial muscles regeneration in vivo.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherBlackwell Munksgaard-
dc.relation.isPartOfORTHODONTICS & CRANIOFACIAL RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdolescent-
dc.subject.MESHAzacitidine/analogs & derivatives*-
dc.subject.MESHAzacitidine/pharmacology-
dc.subject.MESHBicuspid-
dc.subject.MESHCell Differentiation*/drug effects-
dc.subject.MESHCells, Cultured-
dc.subject.MESHDNA Methylation-
dc.subject.MESHDental Pulp/cytology*-
dc.subject.MESHHumans-
dc.subject.MESHMasseter Muscle/physiology*-
dc.subject.MESHMuscle Development/physiology*-
dc.subject.MESHMuscle, Skeletal/physiology-
dc.subject.MESHRegeneration/drug effects-
dc.subject.MESHRegeneration/physiology*-
dc.subject.MESHYoung Adult-
dc.titleLocal myogenic pulp-derived cell injection enhances craniofacial muscle regeneration in vivo-
dc.typeArticle-
dc.publisher.locationEngland-
dc.contributor.collegeCollege of Dentistry-
dc.contributor.departmentDept. of Orthodontics-
dc.contributor.googleauthorJ. E. Jung-
dc.contributor.googleauthorM. J. Song-
dc.contributor.googleauthorS. Shin-
dc.contributor.googleauthorY. J. Choi-
dc.contributor.googleauthorK. H. Kim-
dc.contributor.googleauthorC. J. Chung-
dc.identifier.doi10.1111/ocr.12138-
dc.contributor.localIdA04139-
dc.contributor.localIdA02117-
dc.contributor.localIdA03724-
dc.contributor.localIdA00309-
dc.relation.journalcodeJ02447-
dc.identifier.eissn1601-6343-
dc.identifier.pmid28102011-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1111/ocr.12138/abstract-
dc.subject.keyword5-aza-2′-deoxycytidine-
dc.subject.keywordmuscle injury-
dc.subject.keywordmyogenesis-
dc.subject.keywordpulp cell-
dc.subject.keywordregeneration-
dc.contributor.alternativeNameKim, Kyung Ho-
dc.contributor.alternativeNameChoi, Yoon Jeong-
dc.contributor.alternativeNameShin, Su Jung-
dc.contributor.alternativeNameChung, Choo Ryung-
dc.contributor.affiliatedAuthorChoi, Yoon Jeong-
dc.contributor.affiliatedAuthorShin, Su Jung-
dc.contributor.affiliatedAuthorChung, Choo Ryung-
dc.contributor.affiliatedAuthorKim, Kyung Ho-
dc.citation.titleOrthodontics & Craniofacial Research-
dc.citation.volume20-
dc.citation.number1-
dc.citation.startPage35-
dc.citation.endPage43-
dc.identifier.bibliographicCitationORTHODONTICS & CRANIOFACIAL RESEARCH, Vol.20(1) : 35-43, 2017-
dc.date.modified2017-11-01-
dc.identifier.rimsid41562-
dc.type.rimsART-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Orthodontics (교정과학교실) > 1. Journal Papers

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