Cited 9 times in
Local myogenic pulp-derived cell injection enhances craniofacial muscle regeneration in vivo
DC Field | Value | Language |
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dc.contributor.author | 김경호 | - |
dc.contributor.author | 최윤정 | - |
dc.contributor.author | 신수정 | - |
dc.contributor.author | 정주령 | - |
dc.date.accessioned | 2017-11-02T08:05:41Z | - |
dc.date.available | 2017-11-02T08:05:41Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 1601-6335 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/154068 | - |
dc.description.abstract | OBJECTIVES: To enhance myogenic differentiation in pulp cells isolated from extracted premolars by epigenetic modification using a DNA demethylation agent, 5-aza-2'-deoxycytidine (5-Aza), and to evaluate the potent stimulatory effect of 5-Aza-treated pulp cell injection for craniofacial muscle regeneration in vivo. SETTING AND SAMPLE POPULATION: Pulp cells were isolated from premolars extracted for orthodontic purposes from four adults (age range, 18-22.1 years). MATERIAL AND METHODS: Levels of myogenic differentiation and functional contraction response in vitro were compared between pulp cells with or without pre-treatment of 5-Aza. Changes in muscle regeneration in response to green fluorescent protein (GFP)-labelled myogenic pulp cell injection in vivo were evaluated using a cardiotoxin (CTX)-induced muscle injury model of the gastrocnemius as well as the masseter muscle in mice. RESULTS: Pre-treatment of 5-Aza in pulp cells stimulated myotube formation, myogenic differentiation in terms of desmin and myogenin expression, and the level of collagen gel contraction. The local injection of 5-Aza pre-treated myogenic pulp cells was engrafted into the host tissue and indicated signs of enhanced muscle regeneration in both the gastrocnemius and the masseter muscles. CONCLUSION: The epigenetic modification of pulp cells from extracted premolars and the local injection of myogenic pulp cells may stimulate craniofacial muscles regeneration in vivo. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Blackwell Munksgaard | - |
dc.relation.isPartOf | ORTHODONTICS & CRANIOFACIAL RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adolescent | - |
dc.subject.MESH | Azacitidine/analogs & derivatives* | - |
dc.subject.MESH | Azacitidine/pharmacology | - |
dc.subject.MESH | Bicuspid | - |
dc.subject.MESH | Cell Differentiation*/drug effects | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | DNA Methylation | - |
dc.subject.MESH | Dental Pulp/cytology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Masseter Muscle/physiology* | - |
dc.subject.MESH | Muscle Development/physiology* | - |
dc.subject.MESH | Muscle, Skeletal/physiology | - |
dc.subject.MESH | Regeneration/drug effects | - |
dc.subject.MESH | Regeneration/physiology* | - |
dc.subject.MESH | Young Adult | - |
dc.title | Local myogenic pulp-derived cell injection enhances craniofacial muscle regeneration in vivo | - |
dc.type | Article | - |
dc.publisher.location | England | - |
dc.contributor.college | College of Dentistry | - |
dc.contributor.department | Dept. of Orthodontics | - |
dc.contributor.googleauthor | J. E. Jung | - |
dc.contributor.googleauthor | M. J. Song | - |
dc.contributor.googleauthor | S. Shin | - |
dc.contributor.googleauthor | Y. J. Choi | - |
dc.contributor.googleauthor | K. H. Kim | - |
dc.contributor.googleauthor | C. J. Chung | - |
dc.identifier.doi | 10.1111/ocr.12138 | - |
dc.contributor.localId | A04139 | - |
dc.contributor.localId | A02117 | - |
dc.contributor.localId | A03724 | - |
dc.contributor.localId | A00309 | - |
dc.relation.journalcode | J02447 | - |
dc.identifier.eissn | 1601-6343 | - |
dc.identifier.pmid | 28102011 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/ocr.12138/abstract | - |
dc.subject.keyword | 5-aza-2′-deoxycytidine | - |
dc.subject.keyword | muscle injury | - |
dc.subject.keyword | myogenesis | - |
dc.subject.keyword | pulp cell | - |
dc.subject.keyword | regeneration | - |
dc.contributor.alternativeName | Kim, Kyung Ho | - |
dc.contributor.alternativeName | Choi, Yoon Jeong | - |
dc.contributor.alternativeName | Shin, Su Jung | - |
dc.contributor.alternativeName | Chung, Choo Ryung | - |
dc.contributor.affiliatedAuthor | Choi, Yoon Jeong | - |
dc.contributor.affiliatedAuthor | Shin, Su Jung | - |
dc.contributor.affiliatedAuthor | Chung, Choo Ryung | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Ho | - |
dc.citation.title | Orthodontics & Craniofacial Research | - |
dc.citation.volume | 20 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 35 | - |
dc.citation.endPage | 43 | - |
dc.identifier.bibliographicCitation | ORTHODONTICS & CRANIOFACIAL RESEARCH, Vol.20(1) : 35-43, 2017 | - |
dc.date.modified | 2017-11-01 | - |
dc.identifier.rimsid | 41562 | - |
dc.type.rims | ART | - |
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