0 160

Cited 13 times in

Environmental enrichment enhances synaptic plasticity by internalization of striatal dopamine transporters

Authors
 Myung-Sun Kim  ;  Ji Hea Yu  ;  Chul Hoon Kim  ;  Jae Yong Choi  ;  Jung Hwa Seo  ;  Min-Young Lee  ;  Chi Hoon Yi  ;  Tae Hyun Choi  ;  Young Hoon Ryu  ;  Jong Eun Lee  ;  Bae Hwan Lee  ;  Hyongbum Kim  ;  Sung-Rae Cho 
Citation
 JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, Vol.36(12) : 2122-2133, 2016 
Journal Title
 JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM 
ISSN
 0271-678X 
Issue Date
2016
MeSH
Animals ; Corpus Striatum/chemistry ; Dopamine Plasma Membrane Transport Proteins/metabolism* ; Endocytosis ; Environment* ; Mice ; Neuronal Plasticity* ; Phosphorylation ; Protein Kinase C/metabolism
Keywords
Dopamine transporter ; environmental enrichment ; internalization ; phosphorylation
Abstract
Environmental enrichment (EE) with a complex combination of physical, cognitive and social stimulations enhances synaptic plasticity and behavioral function. However, the mechanism remains to be elucidated in detail. We aimed to investigate dopamine-related synaptic plasticity underlying functional improvement after EE. For this, six-week-old CD-1 mice were randomly allocated to EE or standard conditions for two months. EE significantly enhanced behavioral functions such as rotarod and ladder walking tests. In a [18F]FPCIT positron emission tomography scan, binding values of striatal DAT were significantly decreased approximately 18% in the EE mice relative to the control mice. DAT inhibitor administrated to establish the relationship of the DAT down-regulation to the treatment effects also improved rotarod performances, suggesting that DAT inhibition recapitulated EE-mediated treatment benefits. Next, EE-induced internalization of DAT was confirmed using a surface biotinylation assay. In situ proximity ligation assay and immunoprecipitation demonstrated that EE significantly increased the phosphorylation of striatal DAT as well as the levels of DAT bound with protein kinase C (PKC). In conclusion, we suggest that EE enables phosphorylation of striatal DAT via a PKC-mediated pathway and causes DAT internalization. This is the first report to suggest an EE-mediated mechanism of synaptic plasticity by internalization of striatal DAT.
Full Text
http://journals.sagepub.com/doi/abs/10.1177/0271678X15613525
DOI
10.1177/0271678X15613525
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Nuclear Medicine (핵의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Rehabilitation Medicine (재활의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chul Hoon(김철훈) ORCID logo https://orcid.org/0000-0002-7360-429X
Kim, Hyongbum(김형범) ORCID logo https://orcid.org/0000-0002-4693-738X
Ryu, Young Hoon(유영훈) ORCID logo https://orcid.org/0000-0002-9000-5563
Lee, Bae Hwan(이배환) ORCID logo https://orcid.org/0000-0003-4719-9021
Lee, Jong Eun(이종은) ORCID logo https://orcid.org/0000-0001-6203-7413
Cho, Sung-Rae(조성래) ORCID logo https://orcid.org/0000-0003-1429-2684
Choi, Ja Young(최자영)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/152532
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse