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Comparison of the oral microbiome of siblings using next-generation sequencing: a pilot study

Authors
 H-S Lee  ;  JH Lee  ;  S-O Kim  ;  JS Song  ;  BI Kim  ;  YJ Kim  ;  J-H Lee 
Citation
 ORAL DISEASES, Vol.22(6) : 549-556, 2016 
Journal Title
 ORAL DISEASES 
ISSN
 1354-523X 
Issue Date
2016
MeSH
Dental Caries/microbiology ; Humans ; Mouth/microbiology* ; Pilot Projects ; Siblings
Keywords
dental caries ; next-generation sequencing ; oral microbiome ; pyrosequencing ; sibling
Abstract
OBJECTIVE: The purpose of this study was to compare the oral microbiome of siblings with and without dental caries using next-generation sequencing. MATERIALS AND METHODS: To investigate the oral microbiome composition, 14 young siblings, seven with caries and seven without, were enrolled from seven sibling-pair families. Supragingival plaque samples were collected from the cervicobuccal area of posterior teeth. All samples were analyzed by pyrosequencing, based on the 16S rRNA gene hypervariable regions, V1-V4. RESULTS: The organisms identified belonged to 65 genera. Fifty-two genera were identified in the subjects with caries and 58 in those without; 45 genera were shared by both groups. In the Shannon index, the caries group showed lower bacterial diversity than the caries-free group and the difference was significant (Wilcoxon signed-rank test, P < 0.05). Additionally, similarities between siblings were evident in analyses based on weighted UniFrac distances (P < 0.05). CONCLUSIONS: In this study, the diversity of the microbiome was reduced in subjects with dental caries, while similarity between siblings seemed to be retained.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/odi.12491/abstract
DOI
10.1111/odi.12491
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Preventive Dentistry and Public Oral Health (예방치과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Baek Il(김백일) ORCID logo https://orcid.org/0000-0001-8234-2327
Kim, Seong Oh(김성오) ORCID logo https://orcid.org/0000-0002-8620-1377
Song, Je Seon(송제선) ORCID logo https://orcid.org/0000-0001-8620-5629
Lee, Jae Ho(이제호) ORCID logo https://orcid.org/0000-0002-1556-3485
Lee, Ji Hyun(이지현)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/151909
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