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Comparative analysis of secretory factors from permanent- and deciduous-teeth periodontal ligament cells

Authors
 Kirim Kim  ;  Mijeong Jeon  ;  Hyo-Seol Lee  ;  Jung-Chul Park  ;  Seok-Jun Moon  ;  Seong-Oh Kim  ;  Sung-Won Cho  ;  Je Seon Song 
Citation
 ARCHIVES OF ORAL BIOLOGY, Vol.71 : 65-79, 2016 
Journal Title
 ARCHIVES OF ORAL BIOLOGY 
ISSN
 0003-9969 
Issue Date
2016
MeSH
Adolescent ; Blotting, Western ; Cells, Cultured ; Child ; Child, Preschool ; Chromatography, High Pressure Liquid ; Culture Media, Conditioned ; Cytokines/immunology* ; Cytokines/metabolism* ; Dentition, Permanent ; Electrophoresis, Polyacrylamide Gel ; Female ; Humans ; Infant ; Male ; Periodontal Ligament/cytology* ; Periodontal Ligament/metabolism* ; Spectrometry, Mass, Electrospray Ionization ; Tooth, Deciduous
Keywords
Cytokine ; Deciduous teeth ; Periodontal ligament cells ; Permanent teeth ; Secretory factors
Abstract
OBJECTIVE: Studies of regenerative therapies have focused on the paracrine effects of mesenchymal stem cells, but little has been revealed about the humoral factors of periodontal ligament (PDL) stem cells. The aim of this study was to identify and compare the secretory factors of human permanent- and deciduous-teeth PDL cells (P-PDL and D-PDL cells, respectively) in order to understand the characteristics of these cells and their potential applications in regenerative therapies. DESIGN: Conditioned media were collected from P-PDL and D-PDL cells (P-PDL-CM and D-PDL-CM, respectively). These media were analyzed with high-performance liquid-chromatography-coupled electrospray ionization tandem mass spectrometry and a cytokine membrane assay. In addition, Western blot analysis was performed to verify the differences between the two media. RESULTS: Cytokines related to neurogenesis (NT-3 and NT-4) and angiogenesis-related cytokines (EGF and IGF-1) were identified in P-PDL-CM. The expression levels of immune-response-related cytokines (interleukins I, II, and IV) and secreted proteins related to tissue degradation and catalytic activities (matrix metallopeptidase 1 (MMP1), Proteasome subunit, alpha type, 1 (PSMA1), and cullin 7 (CUL7)) were higher in D-PDL-CM. Vasorin (VASN) was expressed more strongly in P-PDL-CM, but tudor domain containing 7 (TDRD7) was expressed more strongly in D-PDL-CM in Western blot analysis. CONCLUSION: The cytokine expressions of the two cell types showed different patterns, especially in neurogenesis and immune responses. P-PDL cells are more suitable candidates for applications in regenerative therapies.
Full Text
http://www.sciencedirect.com/science/article/pii/S0003996916301716
DOI
10.1016/j.archoralbio.2016.07.003
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ki Rim(김기림)
Kim, Seong Oh(김성오) ORCID logo https://orcid.org/0000-0002-8620-1377
Moon, Seok Jun(문석준) ORCID logo https://orcid.org/0000-0001-7282-2888
Park, Jung Chul(박정철)
Song, Je Seon(송제선) ORCID logo https://orcid.org/0000-0001-8620-5629
Jeon, Mi Jeong(전미정)
Cho, Sung Won(조성원) ORCID logo https://orcid.org/0000-0001-7505-9769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/151726
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