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Gene copy number change events at chromosome 20 and their association with recurrence in gastric cancer patients

 Sang Hwa Yang  ;  Min Young Seo  ;  Ha Jin Jeong  ;  Hei-Cheul Jeung  ;  Jihye Shin  ;  Sang Chul Kim  ;  Sung Hoon Noh  ;  Hyun Cheol Chung  ;  Sun Young Rha 
 CLINICAL CANCER RESEARCH, Vol.11(2pt1) : 612-620, 2005 
Journal Title
Issue Date
Aged ; Biomarkers, Tumor/genetics* ; Biomarkers, Tumor/metabolism ; Chromosomes, Human, Pair 20/genetics* ; Female ; Gastric Mucosa/metabolism ; Gene Dosage* ; Gene Expression Profiling ; Humans ; Lymphatic Metastasis/pathology ; Male ; Neoplasm Recurrence, Local/genetics* ; Neoplasm Recurrence, Local/metabolism ; Nucleic Acid Hybridization ; Oligonucleotide Array Sequence Analysis ; Prognosis ; Stomach/pathology ; Stomach Neoplasms/genetics* ; Stomach Neoplasms/metabolism ; Survival Rate
PURPOSE: This study examined the gene copy number change events at chromosome 20 in gastric cancer, and their possible relationship with recurrence using cDNA microarray-based comparative genomic hybridization. EXPERIMENTAL DESIGN: Thirty pairs of gastric tumor and normal gastric tissues were used in the cDNA microarray-based comparative genomic hybridization. The cDNA microarrays containing 17,000 sequence-verified human gene probes were used in a direct comparison design, where genomic DNAs from the normal and tumor tissues were labeled with fluorescent dyes Cy3 and Cy5, respectively, and cohybridized. Genes with log(2) (Cy5/Cy3) > or = 0.58 in at least one case were selected as the amplified genes. In order to search for the association between gene copy number changes and the recurrence status, patients were grouped according to their recurrence status. Gene selection between the two groups was done, and each patient was given a score based on the sum of the selected genes' ratios. Logistic regression analysis was carried out in order to determine if the score of a group of patients was correlated with a recurrence. RESULTS: A group of genes including NCOA6, CYP24A1, PTPN1, and ZNF217 was amplified in gastric cancer. Another group of 39 genes, whose sum of copy number change levels was significantly associated with a poor prognosis for recurrence, was selected (P < 0.05). CONCLUSION: Ninety-six amplified genes at chromosome 20 of gastric cancer are reported. A scoring system based on gene copy changes at chromosome 20 can provide an independent patient grouping system that can distinguish patient recurrence status and survival.
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1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Sang Chul(김상철)
Noh, Sung Hoon(노성훈) ORCID logo https://orcid.org/0000-0003-4386-6886
Rha, Sun Young(라선영) ORCID logo https://orcid.org/0000-0002-2512-4531
Seo, Min Young(서민영)
Shin, Ji Hyun(신지현)
Yang, Sang Hwa(양상화)
Jeong, Ha Jin(정하진)
Chung, Hyun Cheol(정현철) ORCID logo https://orcid.org/0000-0002-0920-9471
Jeung, Hei Cheul(정희철) ORCID logo https://orcid.org/0000-0003-0952-3679
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