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Isolation and characterization of tumorspheres from a recurrent pineoblastoma patient: Feasibility of a patient-derived xenograft.

Authors
 Jiyong Kwak  ;  Jin-Kyoung Shim  ;  Dong Seok Kim  ;  Ji-Hyun Lee  ;  Junjeong Choi  ;  Junseong Park  ;  Kyoung-Jin Shin  ;  Se-Hoon Kim  ;  Pilnam Kim  ;  Yong-Min Huh  ;  Eui Hyun Kim  ;  Jong Hee Chang  ;  Sun Ho Kim  ;  Seok-Gu Ka 
Citation
 INTERNATIONAL JOURNAL OF ONCOLOGY, Vol.49(2) : 567-578, 2016 
Journal Title
INTERNATIONAL JOURNAL OF ONCOLOGY
ISSN
 1019-6439 
Issue Date
2016
MeSH
Animals ; Brain Neoplasms/pathology* ; Child ; Feasibility Studies ; Humans ; Male ; Mice ; Neoplasm Transplantation/methods* ; Pineal Gland/pathology* ; Pinealoma/pathology* ; Transplantation, Heterologous/methods*
Abstract
The existence oftumorspheres(TSs) might confer treatment resistance topineoblastoma(PB). The existence of PB TSs with cellular immortalization potential has not yet been reported. We developed a procedure for isolating TSs fromrecurrentPB (rPB) and tested whether their properties made them suitable for use as apatient-derivedxenograft(PDX). Immunocytochemical staining, RT-PCR and quantitative real-time PCR showed that, among stemness proteins, CD133, musashi and podoplanin were expressed at elevated levels in rPB TSs, but nestin was not. rPB TSs cultured under neuro-glial differentiation conditions expressed TUBB3, but not GFAP, MBP or NeuN. Unlike glioblastoma TSs, rPB TSs showed no clear evidence of invasion in 3D invasion assay or increased expression of genes associated with epithelial-mesenchymal transition. An orthotopicxenograftshowed that tumor xenografts replicated the histopathological features of thepatienttumor and expressed similar genome profiles, as determined by short tandem repeat genotyping. These data demonstrate theisolationand thecharacterizationof rPB TSs for the first time. Using an orthotopicxenograft, we showed that rPB TSs could replicate thepatienttumor, demonstrating their potential as a PDX for precision medicine.
Full Text
https://www.spandidos-publications.com/ijo/49/2/569
DOI
10.3892/ijo.2016.3554
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Kang, Seok Gu(강석구) ORCID logo https://orcid.org/0000-0001-5676-2037
Kwak, Jiyong(곽지용) ORCID logo https://orcid.org/0000-0002-7738-9136
Kim, Dong Seok(김동석)
Kim, Sun Ho(김선호) ORCID logo https://orcid.org/0000-0003-0970-3848
Kim, Se Hoon(김세훈) ORCID logo https://orcid.org/0000-0001-7516-7372
Kim, Eui Hyun(김의현) ORCID logo https://orcid.org/0000-0002-2523-7122
Shin, Kyoung Jin(신경진) ORCID logo https://orcid.org/0000-0002-1059-9665
Lee, Ji Hyun(이지현) ORCID logo https://orcid.org/0000-0002-9223-9478
Chang, Jong Hee(장종희) ORCID logo https://orcid.org/0000-0003-1509-9800
Huh, Yong Min(허용민) ORCID logo https://orcid.org/0000-0002-9831-4475
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146967
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