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Isolation and characterization of tumorspheres from a recurrent pineoblastoma patient: Feasibility of a patient-derived xenograft.

DC FieldValueLanguage
dc.contributor.author강석구-
dc.contributor.author허용민-
dc.contributor.author곽지용-
dc.contributor.author김동석-
dc.contributor.author김선호-
dc.contributor.author김세훈-
dc.contributor.author김의현-
dc.contributor.author신경진-
dc.contributor.author장종희-
dc.contributor.author이지현-
dc.date.accessioned2017-02-27T07:42:29Z-
dc.date.available2017-02-27T07:42:29Z-
dc.date.issued2016-
dc.identifier.issn1019-6439-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/146967-
dc.description.abstractThe existence of tumorspheres (TSs) might confer treatment resistance to pineoblastoma (PB). The existence of PB TSs with cellular immortalization potential has not yet been reported. We developed a procedure for isolating TSs from recurrent PB (rPB) and tested whether their properties made them suitable for use as a patient-derived xenograft (PDX). Immunocytochemical staining, RT-PCR and quantitative real-time PCR showed that, among stemness proteins, CD133, musashi and podoplanin were expressed at elevated levels in rPB TSs, but nestin was not. rPB TSs cultured under neuro-glial differentiation conditions expressed TUBB3, but not GFAP, MBP or NeuN. Unlike glioblastoma TSs, rPB TSs showed no clear evidence of invasion in 3D invasion assay or increased expression of genes associated with epithelial-mesenchymal transition. An orthotopic xenograft showed that tumor xenografts replicated the histopathological features of the patient tumor and expressed similar genome profiles, as determined by short tandem repeat genotyping. These data demonstrate the isolation and the characterization of rPB TSs for the first time. Using an orthotopic xenograft, we showed that rPB TSs could replicate the patient tumor, demonstrating their potential as a PDX for precision medicine.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherD.A. Spandidos-
dc.relation.isPartOfINTERNATIONAL JOURNAL OF ONCOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBrain Neoplasms/pathology*-
dc.subject.MESHChild-
dc.subject.MESHFeasibility Studies-
dc.subject.MESHHumans-
dc.subject.MESHMale-
dc.subject.MESHMice-
dc.subject.MESHNeoplasm Transplantation/methods*-
dc.subject.MESHPineal Gland/pathology*-
dc.subject.MESHPinealoma/pathology*-
dc.subject.MESHTransplantation, Heterologous/methods*-
dc.titleIsolation and characterization of tumorspheres from a recurrent pineoblastoma patient: Feasibility of a patient-derived xenograft.-
dc.typeArticle-
dc.publisher.locationGreece-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Neurosurgery-
dc.contributor.googleauthorJiyong Kwak-
dc.contributor.googleauthorJin-Kyoung Shim-
dc.contributor.googleauthorDong Seok Kim-
dc.contributor.googleauthorJi-Hyun Lee-
dc.contributor.googleauthorJunjeong Choi-
dc.contributor.googleauthorJunseong Park-
dc.contributor.googleauthorKyoung-Jin Shin-
dc.contributor.googleauthorSe-Hoon Kim-
dc.contributor.googleauthorPilnam Kim-
dc.contributor.googleauthorYong-Min Huh-
dc.contributor.googleauthorEui Hyun Kim-
dc.contributor.googleauthorJong Hee Chang-
dc.contributor.googleauthorSun Ho Kim-
dc.contributor.googleauthorSeok-Gu Ka-
dc.identifier.doi10.3892/ijo.2016.3554-
dc.contributor.localIdA00036-
dc.contributor.localIdA04359-
dc.contributor.localIdA04511-
dc.contributor.localIdA00402-
dc.contributor.localIdA00560-
dc.contributor.localIdA00610-
dc.contributor.localIdA00837-
dc.contributor.localIdA02085-
dc.contributor.localIdA03470-
dc.contributor.localIdA03218-
dc.relation.journalcodeJ01141-
dc.identifier.eissn1791-2423-
dc.identifier.pmid27277549-
dc.identifier.urlhttps://www.spandidos-publications.com/ijo/49/2/569-
dc.contributor.alternativeNameKang, Seok Gu-
dc.contributor.alternativeNameHuh, Yong Min-
dc.contributor.alternativeNameKwak, Jiyong-
dc.contributor.alternativeNameKim, Dong Seok-
dc.contributor.alternativeNameKim, Sun Ho-
dc.contributor.alternativeNameKim, Se Hoon-
dc.contributor.alternativeNameKim, Eui Hyun-
dc.contributor.alternativeNameShin, Kyoung Jin-
dc.contributor.alternativeNameChang, Jong Hee-
dc.contributor.affiliatedAuthorKang, Seok Gu-
dc.contributor.affiliatedAuthorHuh, Yong Min-
dc.contributor.affiliatedAuthorKwak, Jiyong-
dc.contributor.affiliatedAuthorKim, Dong Seok-
dc.contributor.affiliatedAuthorKim, Sun Ho-
dc.contributor.affiliatedAuthorKim, Se Hoon-
dc.contributor.affiliatedAuthorKim, Eui Hyun-
dc.contributor.affiliatedAuthorShin, Kyoung Jin-
dc.contributor.affiliatedAuthorChang, Jong Hee-
dc.contributor.affiliatedAuthorLee, Ji Hyun-
dc.citation.volume49-
dc.citation.number2-
dc.citation.startPage567-
dc.citation.endPage578-
dc.identifier.bibliographicCitationINTERNATIONAL JOURNAL OF ONCOLOGY, Vol.49(2) : 567-578, 2016-
dc.date.modified2017-02-24-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과) > 1. Journal Papers

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