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Comparative Gene Expression Analysis of the Coronal Pulp and Apical Pulp Complex in Human Immature Teeth

Authors
 Soo-Hyun Kim  ;  Seunghye Kim  ;  Yooseok Shin  ;  Hyo-Seol Lee  ;  Mijeong Jeon  ;  Seong-Oh Kim  ;  Sung-Won Cho  ;  Nikita B. Ruparel  ;  Je Seon Song 
Citation
 JOURNAL OF ENDODONTICS, Vol.42(5) : 752-759, 2016 
Journal Title
 JOURNAL OF ENDODONTICS 
ISSN
 0099-2399 
Issue Date
2016
MeSH
Adolescent ; CX3C Chemokine Receptor 1 ; Calbindin 1/genetics ; Calcium-Binding Proteins/genetics ; Cell Adhesion/genetics ; Child ; Child, Preschool ; Collagen Type XII/genetics ; Dental Pulp/anatomy & histology ; Dental Pulp/cytology ; Dental Pulp/diagnostic imaging ; Dental Pulp/growth & development* ; Extracellular Matrix Proteins/genetics ; Female ; Gene Expression* ; Gene Expression Profiling ; Hedgehog Proteins/genetics ; Homeodomain Proteins/genetics ; Humans ; Immunohistochemistry ; Laminin/genetics ; Male ; Microarray Analysis/methods ; Neurogenesis/genetics ; Odontogenesis/genetics* ; Osteopontin/genetics ; Phosphoproteins/genetics ; RNA/analysis ; Real-Time Polymerase Chain Reaction/methods ; Receptors, Chemokine/genetics ; Receptors, GABA-A/genetics ; Republic of Korea ; Synaptic Transmission/genetics ; Tooth Apex/anatomy & histology ; Tooth Apex/cytology ; Tooth Apex/diagnostic imaging ; Tooth Apex/growth & development* ; Tooth Calcification/genetics ; Transcription Factors/genetics ; Young Adult
Keywords
Apical pulp complex ; complementary DNA microarray ; coronal pulp ; human immature teeth ; immunohistochemical staining
Abstract
INTRODUCTION: This study determined the gene expression profiles of the human coronal pulp (CP) and apical pulp complex (APC) with the aim of explaining differences in their functions. METHODS: Total RNA was isolated from the CP and APC, and gene expression was analyzed using complementary DNA microarray technology. Gene ontology analysis was used to classify the biological function. Quantitative reverse-transcription polymerase chain reaction and immunohistochemical staining were performed to verify microarray data. RESULTS: In the microarray analyses, expression increases of at least 2-fold were present in 125 genes in the APC and 139 genes in the CP out of a total of 33,297 genes. Gene ontology class processes found more genes related to immune responses, cell growth and maintenance, and cell adhesion in the APC, whereas transport and neurogenesis genes predominated in the CP. Quantitative reverse-transcription polymerase chain reaction and immunohistochemical staining confirmed the microarray results, with DMP1, CALB1, and GABRB1 strongly expressed in the CP, whereas SMOC2, SHH, BARX1, CX3CR1, SPP1, COL XII, and LAMC2 were strongly expressed in the APC. CONCLUSIONS: The expression levels of genes related to dentin mineralization, neurogenesis, and neurotransmission are higher in the CP in human immature teeth, whereas those of immune-related and tooth development-related genes are higher in the APC.
Full Text
http://www.sciencedirect.com/science/article/pii/S0099239916000959
DOI
10.1016/j.joen.2016.01.024
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Seong Oh(김성오) ORCID logo https://orcid.org/0000-0002-8620-1377
Song, Je Seon(송제선) ORCID logo https://orcid.org/0000-0001-8620-5629
Shin, Yoo Seok(신유석) ORCID logo https://orcid.org/0000-0003-1121-2570
Cho, Sung Won(조성원) ORCID logo https://orcid.org/0000-0001-7505-9769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146792
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