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Distinctive Genetic Activity Pattern of the Human Dental Pulp between Deciduous and Permanent Teeth

Authors
 Ji-Hee Kim  ;  Mijeong Jeon  ;  Je-Seon Song  ;  Jae-Ho Lee  ;  Byung-Jai Choi  ;  Han-Sung Jung  ;  Seok Jun Moon  ;  Pamela K. DenBesten  ;  Seong-Oh Kim 
Citation
 PLOS ONE, Vol.9(7) : e102893, 2014 
Journal Title
 PLOS ONE 
Issue Date
2014
MeSH
Adolescent ; Calbindin 1/genetics ; Calbindin 1/metabolism ; Child ; Dental Pulp/metabolism* ; Female ; Gene Expression Profiling ; Humans ; Male ; RNA-Binding Proteins/genetics ; RNA-Binding Proteins/metabolism ; Receptors, G-Protein-Coupled/genetics ; Receptors, G-Protein-Coupled/metabolism ; Receptors, GABA-A/genetics ; Receptors, GABA-A/metabolism ; Tooth/metabolism* ; Tooth, Deciduous/metabolism
Abstract
Human deciduous and permanent teeth exhibit different developmental processes, morphologies, histological characteristics and life cycles. In addition, their pulp tissues react differently to external stimuli, such as the pulp sensitivity test, dental trauma and pulp therapy materials. These suggest differences in gene expression and regulation, and in this study we compared gene-expression profiles of the human dental pulp from deciduous and permanent teeth. Pulp tissues from permanent premolars and deciduous molars aged 11–14 years were extirpated and mRNA was isolated for cDNA microarray analysis, and quantitative real-time PCR (qPCR). Other teeth were used for immunohistochemical analysis (IHC). Microarray analysis identified 263 genes with a twofold or greater difference in expression level between the two types of pulp tissue, 43 and 220 of which were more abundant in deciduous and permanent pulp tissues, respectively. qPCR analysis was conducted for eight randomly selected genes, and the findings were consistent with the cDNA microarray results. IHC confirmed that insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) was broadly expressed in deciduous dental pulp tissue, but minimally expressed in permanent dental pulp tissue. Immunohistochemical analysis showed that calbindin 1 (CALB1), leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5), and gamma-aminobutyric acid A receptor beta 1 (GABRB1) were abundantly expressed in permanent predentin/odontoblasts, but only minimally expressed in deciduous dental pulp tissue. These results show that deciduous and permanent pulp tissues have different characteristics and gene expression, suggesting that they may have different functions and responses to therapies focused on pulp or dentin regeneration.
Files in This Item:
T201402167.pdf Download
DOI
10.1371/journal.pone.0102893
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Oral Science Research Center (구강과학연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Seong Oh(김성오) ORCID logo https://orcid.org/0000-0002-8620-1377
Moon, Seok Jun(문석준) ORCID logo https://orcid.org/0000-0001-7282-2888
Song, Je Seon(송제선) ORCID logo https://orcid.org/0000-0001-8620-5629
Lee, Jae Ho(이제호) ORCID logo https://orcid.org/0000-0002-1556-3485
Jeon, Mi Jeong(전미정)
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
Choi, Byung Jai(최병재)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/99182
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