2 421

Cited 13 times in

In vitro and in vivo characteristics of stem cells from human exfoliated deciduous teeth obtained by enzymatic disaggregation and outgrowth

 Mijeong Jeon  ;  Je Seon Song  ;  Byung-Jai Choi  ;  Hyung-Jun Choi  ;  Dong-Min Shin  ;  Han-Sung Jung  ;  Seong-Oh Kim 
 ARCHIVES OF ORAL BIOLOGY, Vol.59(10) : 1013-1023, 2014 
Journal Title
Issue Date
Alkaline Phosphatase/analysis ; Animals ; Cell Differentiation/physiology ; Cell Proliferation/physiology ; Child ; Child, Preschool ; Female ; Flow Cytometry ; Humans ; In Vitro Techniques ; Male ; Mice ; Mice, Inbred BALB C ; Osteogenesis/physiology ; Real-Time Polymerase Chain Reaction ; Regeneration/physiology ; Regenerative Medicine ; Staining and Labeling ; Stem Cell Transplantation* ; Stem Cells/cytology* ; Stem Cells/enzymology ; Tooth Exfoliation ; Tooth, Deciduous/cytology*
Enzymatic disaggregation ; Hard tissues ; In vivo transplantation ; Outgrowth ; Stem cells from human exfoliated deciduous teeth (SHED)
OBJECTIVE: Stem cells from human exfoliated deciduous teeth (SHED) are a good source of dental tissue for regeneration therapy, and can be obtained using different primary culture methods. The aim of this study was to determine the differences in the in vitro and in vivo characteristics between SHED isolated via enzymatic disaggregation (e-SHED) and outgrowth (o-SHED) primary culture methods. DESIGN: Dental pulp stem cells were isolated from 14 exfoliated deciduous teeth by enzymatic disaggregation (n=7) and outgrowth (n=7). Their proliferation potential and colony-forming ability were evaluated in vitro, as was their mesenchymal stem-cell-marker expression (using flow cytometry), and their differentiation was verified using quantitative real-time PCR (qPCR) and histochemical staining. In addition, the qualitative and quantitative characteristics of the hard tissue that was generated after in vivo transplantation were compared using haematoxylin and eosin staining, immunohistochemical staining, qPCR, and quantitative alkaline phosphatase analysis. RESULTS: The cell-proliferation potential, colony-forming ability, and Stro-1 and CD146 expression were higher in e-SHED than in o-SHED. While the in vitro adipogenic differentiation potential was greater in e-SHED than in o-SHED, the in vitro osteogenic differentiation did not differ significantly between the two cell types. Although in vivo hard tissue formation was greater following transplantation of o-SHED into mice, there was no difference in the quality of hard tissue generated by e-SHED and o-SHED. CONCLUSION: The findings of this study indicate that e-SHED exhibit stronger stemness characteristics, but that o-SHED are more suitable for hard-tissue regeneration therapy in teeth.
Full Text
Appears in Collections:
5. Research Institutes (연구소) > Oral Science Research Center (구강과학연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Seong Oh(김성오) ORCID logo https://orcid.org/0000-0002-8620-1377
Song, Je Seon(송제선) ORCID logo https://orcid.org/0000-0001-8620-5629
Shin, Dong Min(신동민) ORCID logo https://orcid.org/0000-0001-6042-0435
Jeon, Mi Jeong(전미정)
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
Choi, Byung Jai(최병재)
Choi, Hyung Jun(최형준) ORCID logo https://orcid.org/0000-0002-3315-6912
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.