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탈락 유치 내 치수 세포의 보관 용액과 기간에 따른 생존

Other Titles
 Effect of Storage Media and Duration on Pulpal Cell Viability in Exfoliated Deciduous Teeth 
Authors
 Jiwon Park  ;  Jeseon Song  ;  Jaeho Lee  ;  Seongoh Kim  ;  Mijeong Jeon  ;  Hansung Jung  ;  Heungkyu Son 
Citation
 Journal of the Korean Academy of Pediatric Dentistry (대한소아치과학회지), Vol.41(1) : 1-7, 2014 
Journal Title
 Journal of the Korean Academy of Pediatric Dentistry  (대한소아치과학회지) 
ISSN
 1226-8496 
Issue Date
2014
Abstract
If it is possible to preserve and culture cells from exfoliated deciduous teeth in a readily available storage medium within each family, more stem cells would be obtained. This research is about the effect of storage media and time on pulpal cell viability of exfoliated deciduous teeth. 330 exfoliated deciduous teeth were randomly divided into 11 groups; fresh group, dry group, groups stored in cell culture medium (2, 4, 7 days each), in milk (2, 4, 7 days each), and in saline (2, 4, 7 days each). Primary culture of pulpal cells was conducted in each group and the success rates were compared by calculating the number of teeth with viable cells. The result of primary culture shows that the success rate decreases as the time of storage gets longer. There was no statistical difference between groups stored in the cell culture medium, milk, and saline for 2 and 4 days. However, the groups stored in milk and saline for 7 days showed dramatic decrease in success rate compared to the group stored in the cell culture medium. In conclusion, exfoliated or extracted deciduous teeth can be used to culture pulpal cells when they are stored in milk and saline for a certain period of time; however obtaining viable pulpal cells becomes harder as the storage time gets longer.
Files in This Item:
T201400557.pdf Download
DOI
10.5933/JKAPD.2014.41.1.1
Appears in Collections:
5. Research Institutes (연구소) > Oral Science Research Center (구강과학연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
김성오(Kim, Seong Oh) ORCID logo https://orcid.org/0000-0002-8620-1377
손흥규(Son, Heung Kyu)
송제선(Song, Je Seon) ORCID logo https://orcid.org/0000-0001-8620-5629
이제호(Lee, Jae Ho) ORCID logo https://orcid.org/0000-0002-1556-3485
전미정(Jeon, Mi Jeong)
정한성(Jung, Han Sung)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/98279
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