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탈락 유치 내 치수 세포의 보관 용액과 기간에 따른 생존

Other Titles
 Effect of Storage Media and Duration on Pulpal Cell Viability in Exfoliated Deciduous Teeth 
Authors
 Jiwon Park  ;  Jeseon Song  ;  Jaeho Lee  ;  Seongoh Kim  ;  Mijeong Jeon  ;  Hansung Jung  ;  Heungkyu Son 
Citation
 Journal of the Korean Academy of Pediatric Dentistry (대한소아치과학회지), Vol.41(1) : 1-7, 2014 
Journal Title
Journal of the Korean Academy of Pediatric Dentistry(대한소아치과학회지)
ISSN
 1226-8496 
Issue Date
2014
Keywords
Storage medium for deciduous teeth ; Pulpal tissue ; Cell viability ; Milk ; Saline
Abstract
If it is possible to preserve and culture cells from exfoliated deciduous teeth in a readily available storage medium within each family, more stem cells would be obtained. This research is about the effect of storage media and time on pulpal cell viability of exfoliated deciduous teeth. 330 exfoliated deciduous teeth were randomly divided into 11 groups; fresh group, dry group, groups stored in cell culture medium (2, 4, 7 days each), in milk (2, 4, 7 days each), and in saline (2, 4, 7 days each). Primary culture of pulpal cells was conducted in each group and the success rates were compared by calculating the number of teeth with viable cells. The result of primary culture shows that the success rate decreases as the time of storage gets longer. There was no statistical difference between groups stored in the cell culture medium, milk, and saline for 2 and 4 days. However, the groups stored in milk and saline for 7 days showed dramatic decrease in success rate compared to the group stored in the cell culture medium. In conclusion, exfoliated or extracted deciduous teeth can be used to culture pulpal cells when they are stored in milk and saline for a certain period of time; however obtaining viable pulpal cells becomes harder as the storage time gets longer.
Files in This Item:
T201400557.pdf Download
DOI
10.5933/JKAPD.2014.41.1.1
Appears in Collections:
2. College of Dentistry (치과대학) > Research Institute (부설연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Seong Oh(김성오) ORCID logo https://orcid.org/0000-0002-8620-1377
Son, Heung Kyu(손흥규)
Song, Je Seon(송제선) ORCID logo https://orcid.org/0000-0001-8620-5629
Lee, Jae Ho(이제호) ORCID logo https://orcid.org/0000-0002-1556-3485
Jeon, Mi Jeong(전미정)
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/98279
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