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Retinoic acid modulates chondrogenesis in the developing mouse cranial base

Authors
 Hyuk-Jae Kwon  ;  Jeong-Oh Shin  ;  Jong-Min Lee  ;  Kyoung-Won Cho  ;  Min-Jung Lee  ;  Sung-Won Cho  ;  Han-Sung Jung 
Citation
 JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION, Vol.316(8) : 574-583, 2011 
Journal Title
JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION
ISSN
 1552-5007 
Issue Date
2011
MeSH
Animals ; Apoptosis/drug effects ; Apoptosis/physiology ; Bone Morphogenetic Protein 4/drug effects ; Bone Morphogenetic Protein 4/metabolism* ; Cell Differentiation/drug effects ; Cell Differentiation/physiology ; Cell Proliferation/drug effects ; Chondrocytes/cytology* ; Chondrocytes/drug effects ; Chondrogenesis/drug effects ; Chondrogenesis/physiology* ; Integrin-Binding Sialoprotein/drug effects ; Integrin-Binding Sialoprotein/metabolism* ; Ki-67 Antigen/drug effects ; Ki-67 Antigen/metabolism ; Mice ; Monoterpenes/pharmacology ; Organ Culture Techniques ; Osteogenesis/drug effects ; Osteogenesis/physiology ; Receptors, Retinoic Acid/metabolism ; Signal Transduction ; Skull Base/cytology ; Skull Base/drug effects ; Skull Base/embryology* ; Skull Base/metabolism ; Tretinoin/metabolism* ; Tretinoin/pharmacology
Abstract
The retinoic acid (RA) signaling pathway is known to play important roles during craniofacial development and skeletogenesis. However, the specific mechanism involving RA in cranial base development has not yet been clearly described. This study investigated how RA modulates endochondral bone development of the cranial base by monitoring the RA receptor RARγ, BMP4, and markers of proliferation, programmed cell death, chondrogenesis, and osteogenesis. We first examined the dynamic morphological and molecular changes in the sphenooccipital synchondrosis-forming region in the mouse embryo cranial bases at E12-E16. In vitro organ cultures employing beads soaked in RA and retinoid-signaling inhibitor citral were compared. In the RA study, the sphenooccipital synchondrosis showed reduced cartilage matrix and lower BMP4 expression while hypertrophic chondrocytes were replaced with proliferating chondrocytes. Retardation of chondrocyte hypertrophy was exhibited in citral-treated specimens, while BMP4 expression was slightly increased and programmed cell death was induced within the sphenooccipital synchondrosis. Our results demonstrate that RA modulates chondrocytes to proliferate, differentiate, or undergo programmed cell death during endochondral bone formation in the developing cranial base.
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/jez.b.21432/abstract
DOI
10.1002/jez.b.21432
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Others (기타) > 1. Journal Papers
Yonsei Authors
Kwon, Hyuk Jae(권혁제)
Shin, Jeong Oh(신정오) ORCID logo https://orcid.org/0000-0002-6935-0936
Lee, Min Jung(이민정)
Lee, Jong Min(이종민) ORCID logo https://orcid.org/0000-0002-9466-7644
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
Cho, Kyoung Won(조경원)
Cho, Sung Won(조성원) ORCID logo https://orcid.org/0000-0001-7505-9769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/94588
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