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Induction of bone formation by Escherichia coli-expressed recombinant human bone morphogenetic protein-2 using block-type macroporous biphasic calcium phosphate in orthotopic and ectopic rat mod

 J-C. Park  ;  S-S. So  ;  C-S. Kim  ;  K-S. Cho  ;  S-H. Choi  ;  J-H. Yun  ;  I-H. Jung 
 Journal of Periodontal Research, Vol.46(6) : 682-690, 2011 
Journal Title
 Journal of Periodontal Research 
Issue Date
BACKGROUND AND OBJECTIVE: The potential of the Escherichia coli-expressed recombinant human bone morphogenetic protein-2 (ErhBMP-2) to support new bone formation/maturation using a block-type of macroporous biphasic calcium phosphate (bMBCP) carrier was evaluated in an orthotopic and ectopic rat model. MATERIAL AND METHODS: Critical-size (Φ 8 mm) calvarial defects and subcutaneous pockets in 32 Sprague-Dawley rats received implants of rhBMP-2 (2.5 μg) in a bMBCP carrier or bMBCP alone (control). Implant sites were evaluated using histological and histometric analysis following 2- and 8-wk healing intervals (eight animals/group/interval). Results:  ErhBMP-2/bMBCP supported significantly greater bone formation at 2 and 8 wk (10.8% and 25.4%, respectively) than the control at 2 and 8 wk (5.3% and 14.0%, respectively) in calvarial defects (p < 0.01). Bone formation was only observed for the ErhBMP-2/bMBCP ectopic sites and was significantly greater at 8 wk (7.5%) than at 2 wk (4.5%) (p < 0.01). Appositional and endochondral bone formation was usually associated with a significant increase in fatty marrow at 8 wk. The bMBCP carrier showed no evidence of bioresorption. CONCLUSION: ErhBMP-2/bMBCP induced significant bone formation in both calvarial and ectopic sites. Further study appears to be required to evaluate the relevance of the bMBCP carrier.
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1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Periodontology (치주과학교실)
Yonsei Authors
김창성(Kim, Chang Sung) ; 박정철(Park, Jung Chul) ; 정임희(Jung, Im Hee) ; 조규성(Cho, Kyoo Sung) ; 최성호(Choi, Seong Ho)
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