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Induction of bone formation by Escherichia coli-expressed recombinant human bone morphogenetic protein-2 using block-type macroporous biphasic calcium phosphate in orthotopic and ectopic rat mod

Authors
 J-C. Park ; S-S. So ; C-S. Kim ; K-S. Cho ; S-H. Choi ; J-H. Yun ; I-H. Jung 
Citation
 Journal of Periodontal Research, Vol.46(6) : 682~690, 2011 
Journal Title
 Journal of Periodontal Research 
ISSN
 0022-3484 
Issue Date
2011
Abstract
BACKGROUND AND OBJECTIVE: The potential of the Escherichia coli-expressed recombinant human bone morphogenetic protein-2 (ErhBMP-2) to support new bone formation/maturation using a block-type of macroporous biphasic calcium phosphate (bMBCP) carrier was evaluated in an orthotopic and ectopic rat model. MATERIAL AND METHODS: Critical-size (Φ 8 mm) calvarial defects and subcutaneous pockets in 32 Sprague-Dawley rats received implants of rhBMP-2 (2.5 μg) in a bMBCP carrier or bMBCP alone (control). Implant sites were evaluated using histological and histometric analysis following 2- and 8-wk healing intervals (eight animals/group/interval). Results:  ErhBMP-2/bMBCP supported significantly greater bone formation at 2 and 8 wk (10.8% and 25.4%, respectively) than the control at 2 and 8 wk (5.3% and 14.0%, respectively) in calvarial defects (p < 0.01). Bone formation was only observed for the ErhBMP-2/bMBCP ectopic sites and was significantly greater at 8 wk (7.5%) than at 2 wk (4.5%) (p < 0.01). Appositional and endochondral bone formation was usually associated with a significant increase in fatty marrow at 8 wk. The bMBCP carrier showed no evidence of bioresorption. CONCLUSION: ErhBMP-2/bMBCP induced significant bone formation in both calvarial and ectopic sites. Further study appears to be required to evaluate the relevance of the bMBCP carrier.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/94157
DOI
10.1111/j.1600-0765.2011.01390.x
Appears in Collections:
1. 연구논문 > 2. College of Dentistry > Dept. of Periodontology
Yonsei Authors
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Link
 http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0765.2011.01390.x/abstract
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