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Induction of bone formation by Escherichia coli-expressed recombinant human bone morphogenetic protein-2 using block-type macroporous biphasic calcium phosphate in orthotopic and ectopic rat mod

Authors
 J-C. Park  ;  S-S. So  ;  I-H. Jung  ;  J-H. Yun  ;  S-H. Choi  ;  K-S. Cho  ;  C-S. Kim 
Citation
 JOURNAL OF PERIODONTAL RESEARCH, Vol.46(6) : 682-690, 2011 
Journal Title
JOURNAL OF PERIODONTAL RESEARCH
ISSN
 0022-3484 
Issue Date
2011
MeSH
Adipogenesis/drug effects ; Animals ; Bone Morphogenetic Protein 2/biosynthesis ; Bone Morphogenetic Protein 2/pharmacology* ; Bone Substitutes* ; Escherichia coli/metabolism ; Humans ; Hydroxyapatites* ; Male ; Models, Animal ; Osteogenesis/drug effects* ; Porosity ; Rats ; Rats, Sprague-Dawley ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/pharmacology ; Skull/surgery ; Subcutaneous Tissue/surgery ; Tissue Scaffolds*
Keywords
animal model ; bone grafting ; growth factors
Abstract
BACKGROUND AND OBJECTIVE: The potential of the Escherichia coli-expressed recombinant human bone morphogenetic protein-2 (ErhBMP-2) to support new bone formation/maturation using a block-type of macroporous biphasic calcium phosphate (bMBCP) carrier was evaluated in an orthotopic and ectopic rat model.

MATERIAL AND METHODS: Critical-size (Φ 8 mm) calvarial defects and subcutaneous pockets in 32 Sprague-Dawley rats received implants of rhBMP-2 (2.5 μg) in a bMBCP carrier or bMBCP alone (control). Implant sites were evaluated using histological and histometric analysis following 2- and 8-wk healing intervals (eight animals/group/interval). Results:  ErhBMP-2/bMBCP supported significantly greater bone formation at 2 and 8 wk (10.8% and 25.4%, respectively) than the control at 2 and 8 wk (5.3% and 14.0%, respectively) in calvarial defects (p < 0.01). Bone formation was only observed for the ErhBMP-2/bMBCP ectopic sites and was significantly greater at 8 wk (7.5%) than at 2 wk (4.5%) (p < 0.01). Appositional and endochondral bone formation was usually associated with a significant increase in fatty marrow at 8 wk. The bMBCP carrier showed no evidence of bioresorption.

CONCLUSION: ErhBMP-2/bMBCP induced significant bone formation in both calvarial and ectopic sites. Further study appears to be required to evaluate the relevance of the bMBCP carrier.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0765.2011.01390.x/abstract
DOI
10.1111/j.1600-0765.2011.01390.x
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Sung(김창성) ORCID logo https://orcid.org/0000-0003-3902-1071
Park, Jung Chul(박정철)
Jung, Im Hee(정임희)
Cho, Kyoo Sung(조규성) ORCID logo https://orcid.org/0000-0002-6777-5287
Choi, Seong Ho(최성호) ORCID logo https://orcid.org/0000-0001-6704-6124
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/94157
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