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Wnt5a plays a crucial role in determining tooth size during murine tooth development

Authors
 Jinglei Cai  ;  Noriko Mutoh  ;  Jeong-Oh Shin  ;  Nobuyuki Tani-Ishii  ;  Hayato Ohshima  ;  Sung-Won Cho  ;  Han-Sung Jung 
Citation
 CELL AND TISSUE RESEARCH, Vol.345(3) : 367-377, 2011 
Journal Title
CELL AND TISSUE RESEARCH
ISSN
 0302-766X 
Issue Date
2011
MeSH
Amino Acid Chloromethyl Ketones/pharmacology ; Animals ; Apoptosis/drug effects ; Biological Assay ; Body Patterning/drug effects ; Bone Morphogenetic Protein 4/genetics ; Bone Morphogenetic Protein 4/metabolism ; Buffers ; Fibroblast Growth Factor 10/genetics ; Fibroblast Growth Factor 10/metabolism ; Gene Expression Regulation, Developmental/drug effects ; Mice ; Mice, Inbred ICR ; Models, Biological ; Organ Size/drug effects ; Tooth/anatomy & histology* ; Tooth/cytology ; Tooth/drug effects ; Tooth/embryology* ; Tooth Germ/cytology ; Tooth Germ/drug effects ; Tooth Germ/embryology ; Tooth Germ/metabolism ; Wnt Proteins/genetics ; Wnt Proteins/metabolism* ; Wnt Proteins/pharmacology ; Wnt-5a Protein
Keywords
Apoptosis ; Gene expression regulation ; Tooth transplantation ; Wnt proteins ; Mice (ICR)
Abstract
We have previously demonstrated that tooth size is determined by dental mesenchymal factors. Exogenous bone morphogenetic protein (BMP)4, Noggin, fibroblast growth factor (FGF)3 and FGF10 have no effect on tooth size, despite the expressions of Bmp2, Bmp4, Fgf3, Fgf10 and Lef1 in the dental mesenchyme. Among the wingless (Wnt) genes that are differentially expressed during tooth development, only Wnt5a is expressed in the dental mesenchyme. The aims of the present study were to clarify the expression pattern of Wnt5a in developing tooth germs and the role of Wnt5a in the regulation of tooth size by treatment with exogenous WNT5A with/without an apoptosis inhibitor on in vitro tooth germs combined with transplantation into kidney capsules. Wnt5a was intensely expressed in both the dental epithelium and mesenchyme during embryonic days 14-17, overlapping partly with the expressions of both Shh and Bmp4. Moreover, WNT5A retarded the development of tooth germs by markedly inducing cell death in the non-dental epithelium and mesenchyme but not widely in the dental region, where the epithelial-mesenchymal gene interactions among Wnt5a, Fgf10, Bmp4 and Shh might partly rescue the cells from death in the WNT5A-treated tooth germ. Together, these results indicate that WNT5A-induced cell death inhibited the overall development of the tooth germ, resulting in smaller teeth with blunter cusps after tooth-germ transplantation. Thus, it is suggested that Wnt5a is involved in regulating cell death in non-dental regions, while in the dental region it acts as a regulator of other genes that rescue tooth germs from cell death.
Full Text
http://link.springer.com/article/10.1007%2Fs00441-011-1224-4
DOI
10.1007/s00441-011-1224-4
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Shin, Jeong Oh(신정오) ORCID logo https://orcid.org/0000-0002-6935-0936
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
Cho, Sung Won(조성원) ORCID logo https://orcid.org/0000-0001-7505-9769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/93539
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