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과잉치 치주인대 줄기세포의 특성

Other Titles
 Characteristics of Stem Cells Derived from the Periodontal Ligament of Supernumerary Teeth 
 박지현  ;  송제선  ;  김성오  ;  김승혜  ;  최병재  ;  박기호  ;  정한성  ;  이제호 
 Tissue Engineering and Regenerative Medicine (조직공학과 재생의학), Vol.8(suppl 2) : 123-131, 2011 
Journal Title
 Tissue Engineering and Regenerative Medicine  (조직공학과 재생의학) 
Issue Date
Human teeth have been identified as a new source of postnatal stem cells that have the capacities of selfrenewal and multilineage differentiation. In this study we compared the characteristics of stem cells obtained from the periodontal ligament of supernumerary teeth (sPDLSCs) with those of bone-marrow-derived mesenchymal stem cells (BMMSCs), with the aim of extending the sources of stem cells. We performed a colony forming unit-fibroblast (CFU-F) assay to evaluate the self-renewal ability of the cells. Reverse transcription-polymerase chain reaction (RTPCR) and flow cytometry analyses were used to detect the expressions of various stem-cell markers: Oct-4, Nanog, Nestin, Stro-1, CD146, CD105, CD29, CD44, CD90 and CD31. The abilities of adipogenic and osteogenic differentiation were monitored by histochemical staining and quantitative RT-PCR. The colony-forming efficiency was slightly higher for sPDLSCs than for BMMSCs (6.0% vs. 5.1%, respectively). The two stem cell populations expressed similar stem-cell markers except Nestin, Stro-1, and CD146. The sPDLSCs could differentiate to adipogenic lineage; however, the up-regulation of peroxisome-proliferator-activated receptor γ2 (PPARγ2) and lipoprotein lipase (LPL) gene expressions was less than for the BMMSCs. Osteogenic differentiation of the sPDLSCs was confirmed by up-regulation of alkaline phosphatase (ALP) and bone sialoprotein (BSP) gene expressions, but elevation of ALP gene expression was observed earlier than for the BMMSCs. We have shown that sPDLSCs exhibit stem-cell properties such as self-renewal ability, expression of stem-cell markers, and differentiation into adipogenic and osteogenic lineages. This cell type could represent a good stem-cell candidate for use in regenerative medicine.
Appears in Collections:
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실)
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실)
Yonsei Authors
김성오(Kim, Seong Oh) ; 김승혜(Kim, Seung Hye) ; 송제선(Song, Je Seon) ; 이제호(Lee, Jae Ho) ; 정한성(Jung, Han Sung) ; 최병재(Choi, Byung Jai)
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