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Bone formation of Escherichia coli expressed rhBMP-2 on absorbable collagen block in rat calvarial defects

 Seong-Ho Choi  ;  Kyoo-Sung Cho  ;  Chang-Sung Kim  ;  Ui-Won Jung  ;  Yoo-Jung Um  ;  Jeong-Ho Yun  ;  Jee-Hee Jung 
 Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology, Vol.111(3) : 298-305, 2011 
Journal Title
 Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology 
Issue Date
OBJECTIVES: The aim of this study was to evaluate the osteogenic and space-providing effect of rhBMP-2 produced in an Escherichia coli expression system (ErhBMP-2) with absorbable collagen block (ACB) as the carrier system in the rat calvarial defect model. STUDY DESIGN: Eight-millimeter diameter calvarial defects were created in 60 male Sprague-Dawley rats. The animals were divided into 6 groups containing 10 animals each that received sham-surgery control (no material applied), ACB control (ACB alone), or ErhBMP-2/ACB (ACB loaded with 0.025 mg/mL ErhBMP-2). Histological and histometric analysis was performed after 2- and 8-week healing intervals. RESULTS: On histological observation, the level of bone formation in the defects was generally higher at 8 weeks than at 2 weeks. Surgical implantation of ErhBMP-2/ACB resulted in the enhanced bone regeneration compared with controls. Moreover, the collagen remnants of ACB appeared to be completely resorbed in ACB control and ErhBMP-2/ACB group after 8 weeks. Histometic analysis revealed that the ErhBMP-2/ACB group had a significantly greater augmented area, new bone area, and new bone ratio after 2 and 8 weeks than both control groups. However, there was no difference between 2 and 8 weeks in the ErhBMP-2/ACB group in all aspects of the augmented area, new bone area, and new bone ratio (P < .05). CONCLUSION: ErhBMP-2 loaded on ACB can induce favorable bone formation in the rat calvarial defect model, but this collagen carrier in block type did not fulfill the space-maintaining expectations for bone formation by ErhBMP-2.
Appears in Collections:
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Periodontology (치주과학교실)
Yonsei Authors
김창성(Kim, Chang Sung) ; 엄유정(Um, Yoo Jung) ; 윤정호(Yun, Jeong Ho) ; 정의원(Jung, Ui Won) ; 조규성(Cho, Kyoo Sung) ; 최성호(Choi, Seong Ho)
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