132 225

Cited 0 times in

Protease-activated receptor 2 mediates mucus secretion in the airway submucosal gland

Authors
 Hyun Jae Lee  ;  Yu-Mi Yang  ;  Kyubo Kim  ;  Dong Min Shin  ;  Joo-Heon Yoon  ;  Hyung-Ju Cho  ;  Jae Young Choi 
Citation
 PLOS ONE, Vol.7(8) : e43188, 2012 
Journal Title
 PLOS ONE 
Issue Date
2012
MeSH
Adult ; Aged ; Animals ; Calcium/metabolism ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; In Vitro Techniques ; Male ; Mice ; Mice, Knockout ; Middle Aged ; Mucus/secretion* ; Muramidase/drug effects ; Muramidase/metabolism ; Oligopeptides/pharmacology ; Receptor, PAR-2/agonists ; Receptor, PAR-2/metabolism*
Keywords
Adult ; Aged ; Animals ; Calcium/metabolism ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; In Vitro Techniques ; Male ; Mice ; Mice, Knockout ; Middle Aged ; Mucus/secretion* ; Muramidase/drug effects ; Muramidase/metabolism ; Oligopeptides/pharmacology ; Receptor, PAR-2/agonists ; Receptor, PAR-2/metabolism*
Abstract
Protease-activated receptor 2 (PAR2), a G protein-coupled receptor expressed in airway epithelia and smooth muscle, plays an important role in airway inflammation. In this study, we demonstrated that activation of PAR2 induces mucus secretion from the human airway gland and examined the underlying mechanism using the porcine and murine airway glands. The mucosa with underlying submucosal glands were dissected from the cartilage of tissues, pinned with the mucosal side up at the gas/bath solution interface of a physiological chamber, and covered with oil so that secretions from individual glands could be visualized as spherical bubbles in the oil. Secretion rates were determined by optical monitoring of the bubble diameter. The Ca(2+)-sensitive dye Fura2-AM was used to determine intracellular Ca(2+) concentration ([Ca(2+)](i)) by means of spectrofluorometry. Stimulation of human tracheal mucosa with PAR2-activating peptide (PAR2-AP) elevated intracellular Ca(2+) and induced glandular secretion equal to approximately 30% of the carbachol response in the human airway. Porcine gland tissue was more sensitive to PAR2-AP, and this response was dependent on Ca(2+) and anion secretion. When the mouse trachea were exposed to PAR2-AP, large amounts of secretion were observed in both wild type and ΔF508 cystic fibrosis transmembrane conductance regulator mutant mice but there is no secretion from PAR-2 knock out mice. In conclusion, PAR2-AP is an agonist for mucus secretion from the airway gland that is Ca(2+)-dependent and cystic fibrosis transmembrane conductance regulator-independent.
Files in This Item:
T201205186.pdf Download
DOI
22916223
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Research Center for Human Natural Defense System (생체방어연구센터) > 1. Journal Papers
Yonsei Authors
Kim, Kyu Bo(김규보)
Shin, Dong Min(신동민) ORCID logo https://orcid.org/0000-0001-6042-0435
Yang, Yu Mi(양유미)
Yoon, Joo Heon(윤주헌)
Lee, Hyun Jae(이현재)
Cho, Hyung Ju(조형주) ORCID logo https://orcid.org/0000-0002-2851-3225
Choi, Jae Young(최재영) ORCID logo https://orcid.org/0000-0001-9493-3458
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/91735
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse