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Protease-activated receptor 2 mediates mucus secretion in the airway submucosal gland

DC FieldValueLanguage
dc.contributor.author양유미-
dc.contributor.author윤주헌-
dc.contributor.author이현재-
dc.contributor.author조형주-
dc.contributor.author최재영-
dc.contributor.author김규보-
dc.contributor.author신동민-
dc.date.accessioned2014-12-19T17:36:43Z-
dc.date.available2014-12-19T17:36:43Z-
dc.date.issued2012-
dc.identifier.issn1932-6203-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/91735-
dc.description.abstractProtease-activated receptor 2 (PAR2), a G protein-coupled receptor expressed in airway epithelia and smooth muscle, plays an important role in airway inflammation. In this study, we demonstrated that activation of PAR2 induces mucus secretion from the human airway gland and examined the underlying mechanism using the porcine and murine airway glands. The mucosa with underlying submucosal glands were dissected from the cartilage of tissues, pinned with the mucosal side up at the gas/bath solution interface of a physiological chamber, and covered with oil so that secretions from individual glands could be visualized as spherical bubbles in the oil. Secretion rates were determined by optical monitoring of the bubble diameter. The Ca(2+)-sensitive dye Fura2-AM was used to determine intracellular Ca(2+) concentration ([Ca(2+)](i)) by means of spectrofluorometry. Stimulation of human tracheal mucosa with PAR2-activating peptide (PAR2-AP) elevated intracellular Ca(2+) and induced glandular secretion equal to approximately 30% of the carbachol response in the human airway. Porcine gland tissue was more sensitive to PAR2-AP, and this response was dependent on Ca(2+) and anion secretion. When the mouse trachea were exposed to PAR2-AP, large amounts of secretion were observed in both wild type and ΔF508 cystic fibrosis transmembrane conductance regulator mutant mice but there is no secretion from PAR-2 knock out mice. In conclusion, PAR2-AP is an agonist for mucus secretion from the airway gland that is Ca(2+)-dependent and cystic fibrosis transmembrane conductance regulator-independent.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfPLoS One-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdult-
dc.subject.MESHAged-
dc.subject.MESHAnimals-
dc.subject.MESHCalcium/metabolism-
dc.subject.MESHEnzyme-Linked Immunosorbent Assay-
dc.subject.MESHFemale-
dc.subject.MESHHumans-
dc.subject.MESHIn Vitro Techniques-
dc.subject.MESHMale-
dc.subject.MESHMice-
dc.subject.MESHMice, Knockout-
dc.subject.MESHMiddle Aged-
dc.subject.MESHMucus/secretion*-
dc.subject.MESHMuramidase/drug effects-
dc.subject.MESHMuramidase/metabolism-
dc.subject.MESHOligopeptides/pharmacology-
dc.subject.MESHReceptor, PAR-2/agonists-
dc.subject.MESHReceptor, PAR-2/metabolism*-
dc.titleProtease-activated receptor 2 mediates mucus secretion in the airway submucosal gland-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentDept. of Oral Biology (구강생물학)-
dc.contributor.googleauthorHyun Jae Lee-
dc.contributor.googleauthorYu-Mi Yang-
dc.contributor.googleauthorKyubo Kim-
dc.contributor.googleauthorDong Min Shin-
dc.contributor.googleauthorJoo-Heon Yoon-
dc.contributor.googleauthorHyung-Ju Cho-
dc.contributor.googleauthorJae Young Choi-
dc.identifier.doi22916223-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02604-
dc.contributor.localIdA03293-
dc.contributor.localIdA03936-
dc.contributor.localIdA00323-
dc.contributor.localIdA02091-
dc.contributor.localIdA04173-
dc.contributor.localIdA05148-
dc.relation.journalcodeJ02540-
dc.identifier.pmid22916223-
dc.subject.keywordAdult-
dc.subject.keywordAged-
dc.subject.keywordAnimals-
dc.subject.keywordCalcium/metabolism-
dc.subject.keywordEnzyme-Linked Immunosorbent Assay-
dc.subject.keywordFemale-
dc.subject.keywordHumans-
dc.subject.keywordIn Vitro Techniques-
dc.subject.keywordMale-
dc.subject.keywordMice-
dc.subject.keywordMice, Knockout-
dc.subject.keywordMiddle Aged-
dc.subject.keywordMucus/secretion*-
dc.subject.keywordMuramidase/drug effects-
dc.subject.keywordMuramidase/metabolism-
dc.subject.keywordOligopeptides/pharmacology-
dc.subject.keywordReceptor, PAR-2/agonists-
dc.subject.keywordReceptor, PAR-2/metabolism*-
dc.contributor.alternativeNameYang, Yu-Mi-
dc.contributor.alternativeNameYoon, Joo Heon-
dc.contributor.alternativeNameLee, Hyun Jae-
dc.contributor.alternativeNameCho, Hyung Ju-
dc.contributor.alternativeNameChoi, Jae Young-
dc.contributor.alternativeNameKim, Kyu Bo-
dc.contributor.alternativeNameShin, Dong Min-
dc.contributor.affiliatedAuthorYoon, Joo Heon-
dc.contributor.affiliatedAuthorLee, Hyun Jae-
dc.contributor.affiliatedAuthorCho, Hyung Ju-
dc.contributor.affiliatedAuthorKim, Kyu Bo-
dc.contributor.affiliatedAuthorShin, Dong Min-
dc.contributor.affiliatedAuthorChoi, Jae Young-
dc.contributor.affiliatedAuthorYang, Yu Mi-
dc.citation.volume7-
dc.citation.number8-
dc.citation.startPagee43188-
dc.identifier.bibliographicCitationPLoS One, Vol.7(8) : e43188, 2012-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Research Center for Human Natural Defense System (생체방어연구센터) > 1. Journal Papers

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