Animals ; Bone Morphogenetic Protein 2/administration & dosage ; Bone Morphogenetic Protein 2/pharmacology* ; Bone Substitutes/pharmacology* ; Drug Carriers* ; Hydroxyapatites/pharmacology* ; Osteogenesis/drug effects* ; Rats ; Rats, Sprague-Dawley ; Recombinant Proteins ; Skull/diagnostic imaging ; Skull/surgery ; X-Ray Microtomography
Keywords
Animals ; Bone Morphogenetic Protein 2/administration & dosage ; Bone Morphogenetic Protein 2/pharmacology* ; Bone Substitutes/pharmacology* ; Drug Carriers* ; Hydroxyapatites/pharmacology* ; Osteogenesis/drug effects* ; Rats ; Rats, Sprague-Dawley ; Recombinant Proteins ; Skull/diagnostic imaging ; Skull/surgery ; X-Ray Microtomography
Abstract
OBJECTIVE: The aim of the current study was to determine whether a hydroxyapatite (HA)/beta-tricalcium phosphate (β-TCP) ratio of 20/80 impregnated with recombinant human bone morphogenetic protein (rhBMP-2) enhances new bone formation and to evaluate the dose-dependent response of rhBMP-2.
STUDY DESIGN: Critical-sized calvarial defects were made in rats, and biphasic calcium phosphate (BCP) with different rhBMP-2 doses was loaded into rat calvarial defects. The animals were allowed to heal for either 2 or 8 weeks.
RESULTS: The percentages of new bone after 2 and 8 weeks of healing were significantly greater in the rhBMP-2-treated groups (at all doses) than in the control groups. The percentage of remaining BCP was significantly lower at 8 weeks than at 2 weeks in all groups that included BCP.
CONCLUSIONS: rhBMP-2 administered using a BCP carrier significantly induces new bone formation. A dose-dependent response was not shown in the present study.