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Capacitance-based assay for real-time monitoring of endocytosis and cell viability.

Authors
 Rimi Lee  ;  Jihun Kim  ;  Sook Young Kim  ;  Seon Mi Jang  ;  Sun-Mi Lee  ;  In-Hong Choi  ;  Seung Woo Park  ;  Jeon-Soo Shin  ;  Kyung-Hwa Yoo 
Citation
 LAB ON A CHIP, Vol.12(13) : 2377-2384, 2012 
Journal Title
 LAB ON A CHIP 
ISSN
 1473-0197 
Issue Date
2012
MeSH
Antibodies, Monoclonal/immunology ; Antineoplastic Agents/toxicity ; Cell Line, Tumor ; Cell Size ; Cell Survival/drug effects* ; Electric Capacitance* ; Electrochemical Techniques/instrumentation ; Electrochemical Techniques/methods ; Endocytosis/drug effects* ; Humans ; Metal Nanoparticles/chemistry ; Phagocytosis ; Pinocytosis ; Receptor, ErbB-2/immunology ; Receptor, ErbB-2/metabolism ; Silver/chemistry
Keywords
Antibodies, Monoclonal/immunology ; Antineoplastic Agents/toxicity ; Cell Line, Tumor ; Cell Size ; Cell Survival/drug effects* ; Electric Capacitance* ; Electrochemical Techniques/instrumentation ; Electrochemical Techniques/methods ; Endocytosis/drug effects* ; Humans ; Metal Nanoparticles/chemistry ; Phagocytosis ; Pinocytosis ; Receptor, ErbB-2/immunology ; Receptor, ErbB-2/metabolism ; Silver/chemistry
Abstract
Label-free cell-based assays have emerged as a promising means for high-throughput screening. Most label-free sensors are based on impedance measurements that reflect the passive electrical properties of cells. Here we introduce a capacitance-based assay that measures the dielectric constant (capacitance) of biological cells, and demonstrate the feasibility of analyzing endocytosis and screening chemotherapeutic agents with this assay. Endocytosis induces a change in the zeta potential, leading to a change in the dielectric constant which enables real-time endocytosis monitoring using the capacitance sensor. Additionally, since the dielectric constant is proportional to cell radius and cell volume, cell viability can be estimated from the change in capacitance. Therefore, the capacitance sensor array can also be used for cytotoxicity testing for large-scale chemotherapeutic screening.
Full Text
http://pubs.rsc.org/en/Content/ArticleLanding/2012/LC/c2lc21236f
DOI
22522711
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sook Young(김숙영)
Park, Seung Woo(박승우) ORCID logo https://orcid.org/0000-0001-8230-964X
Shin, Jeon Soo(신전수) ORCID logo https://orcid.org/0000-0002-8294-3234
Choi, In Hong(최인홍) ORCID logo https://orcid.org/0000-0001-9851-0137
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/91367
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