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Capacitance-based assay for real-time monitoring of endocytosis and cell viability.

Authors
 Rimi Lee  ;  Jihun Kim  ;  Sook Young Kim  ;  Seon Mi Jang  ;  Sun-Mi Lee  ;  In-Hong Choi  ;  Seung Woo Park  ;  Jeon-Soo Shin  ;  Kyung-Hwa Yoo 
Citation
 Lab on a Chip, Vol.12(13) : 2377-2384, 2012 
Journal Title
 Lab on a Chip 
ISSN
 1473-0197 
Issue Date
2012
Abstract
Label-free cell-based assays have emerged as a promising means for high-throughput screening. Most label-free sensors are based on impedance measurements that reflect the passive electrical properties of cells. Here we introduce a capacitance-based assay that measures the dielectric constant (capacitance) of biological cells, and demonstrate the feasibility of analyzing endocytosis and screening chemotherapeutic agents with this assay. Endocytosis induces a change in the zeta potential, leading to a change in the dielectric constant which enables real-time endocytosis monitoring using the capacitance sensor. Additionally, since the dielectric constant is proportional to cell radius and cell volume, cell viability can be estimated from the change in capacitance. Therefore, the capacitance sensor array can also be used for cytotoxicity testing for large-scale chemotherapeutic screening.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/91367
DOI
10.1039/C2LC21236F
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실)
Yonsei Authors
김숙영(Kim, Sook Young) ; 박승우(Park, Seung Woo) ; 신전수(Shin, Jeon Soo) ; 최인홍(Choi, In Hong)
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Full Text
http://pubs.rsc.org/en/Content/ArticleLanding/2012/LC/c2lc21236f
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