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Acquisition of human alveolar bone-derived stromal cells using minimally irrigated implant osteotomy: in vitro and in vivo evaluations

Authors
 Jung-Chul Park  ;  Jane C. Kim  ;  Yong-Tae Kim  ;  Seong-Ho Choi  ;  Kyoo-Sung Cho  ;  Gun-Il Im  ;  Byung-Soo Kim  ;  Chang-Sung Kim 
Citation
 JOURNAL OF CLINICAL PERIODONTOLOGY, Vol.39(5) : 495-505, 2012 
Journal Title
 JOURNAL OF CLINICAL PERIODONTOLOGY 
ISSN
 0303-6979 
Issue Date
2012
MeSH
Adipocytes/physiology ; Adipogenesis/physiology ; Adult ; Alveolar Process/cytology* ; Animals ; Antigens, Surface/analysis ; Bone Marrow Cells/physiology* ; Calcification, Physiologic/physiology ; Cell Culture Techniques ; Cell Differentiation/physiology ; Cell Proliferation ; Cell Separation ; Cell Transplantation ; Clone Cells/physiology ; Dental Implantation, Endosseous/methods* ; Feasibility Studies ; Female ; Humans ; Male ; Mice ; Mice, Mutant Strains ; Middle Aged ; Osteoblasts/physiology ; Osteogenesis/physiology ; Osteotomy/methods* ; Stromal Cells/physiology* ; Tissue Engineering* ; Transplantation, Heterologous
Keywords
dental implant ; human bone marrow stromal cells ; tissue engineering
Abstract
OBJECTIVES: Implant osteotomy yields a substantial amount of bone in the form of bone chips entrapped within drill flutes, and can provide a promising cell source for tissue engineering. The aims of this study were to isolate human alveolar bone-derived stromal cells (hABCs) obtained during implant osteotomy, and to evaluate osteogenic differentiation capacity of hABCs. MATERIAL AND METHODS: Bone chips were obtained by minimally irrigated implant drilling technique from 10 human donors. Isolated cells were studied with respect to their colony-forming efficiency, surface marker expression by immunofluorescence staining, fluorescence-activated cell sorting analysis and self-renewal potency. To verify the differentiation activity, in vitro osteogenic and adipogenic gene expressions were evaluated by reverse transcription-polymerase chain reaction, and in vitro formation of mineralized nodule and adipocytes was also evaluated. In vivo bone-forming activity was assessed by ectopic transplantation in immunocompromised mice (n = 5). RESULTS: Human alveolar bone-derived stromal cells population with characteristics of mesenchymal stem cells was present in the isolated cells. Upon hABC transplantation, significant ectopic bone formation was induced with the characteristics of fully matured bone tissue. CONCLUSION: The data support the feasibility of using hABCs as a source of stem cells for dentoalveolar bone tissue reconstruction. The cell source has an advantage that the hABCs can be easily acquired during implant surgery.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1600-051X.2012.01865.x/abstract
DOI
22420633
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Sung(김창성) ORCID logo https://orcid.org/0000-0003-3902-1071
Park, Jung Chul(박정철)
Cho, Kyoo Sung(조규성) ORCID logo https://orcid.org/0000-0002-6777-5287
Choi, Seong Ho(최성호) ORCID logo https://orcid.org/0000-0001-6704-6124
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/90706
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