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Acquisition of human alveolar bone-derived stromal cells using minimally irrigated implant osteotomy: in vitro and in vivo evaluations
DC Field | Value | Language |
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dc.contributor.author | 김창성 | - |
dc.contributor.author | 조규성 | - |
dc.contributor.author | 최성호 | - |
dc.contributor.author | 박정철 | - |
dc.date.accessioned | 2014-12-19T17:03:46Z | - |
dc.date.available | 2014-12-19T17:03:46Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0303-6979 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/90706 | - |
dc.description.abstract | OBJECTIVES: Implant osteotomy yields a substantial amount of bone in the form of bone chips entrapped within drill flutes, and can provide a promising cell source for tissue engineering. The aims of this study were to isolate human alveolar bone-derived stromal cells (hABCs) obtained during implant osteotomy, and to evaluate osteogenic differentiation capacity of hABCs. MATERIAL AND METHODS: Bone chips were obtained by minimally irrigated implant drilling technique from 10 human donors. Isolated cells were studied with respect to their colony-forming efficiency, surface marker expression by immunofluorescence staining, fluorescence-activated cell sorting analysis and self-renewal potency. To verify the differentiation activity, in vitro osteogenic and adipogenic gene expressions were evaluated by reverse transcription-polymerase chain reaction, and in vitro formation of mineralized nodule and adipocytes was also evaluated. In vivo bone-forming activity was assessed by ectopic transplantation in immunocompromised mice (n = 5). RESULTS: Human alveolar bone-derived stromal cells population with characteristics of mesenchymal stem cells was present in the isolated cells. Upon hABC transplantation, significant ectopic bone formation was induced with the characteristics of fully matured bone tissue. CONCLUSION: The data support the feasibility of using hABCs as a source of stem cells for dentoalveolar bone tissue reconstruction. The cell source has an advantage that the hABCs can be easily acquired during implant surgery. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | JOURNAL OF CLINICAL PERIODONTOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adipocytes/physiology | - |
dc.subject.MESH | Adipogenesis/physiology | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Alveolar Process/cytology* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antigens, Surface/analysis | - |
dc.subject.MESH | Bone Marrow Cells/physiology* | - |
dc.subject.MESH | Calcification, Physiologic/physiology | - |
dc.subject.MESH | Cell Culture Techniques | - |
dc.subject.MESH | Cell Differentiation/physiology | - |
dc.subject.MESH | Cell Proliferation | - |
dc.subject.MESH | Cell Separation | - |
dc.subject.MESH | Cell Transplantation | - |
dc.subject.MESH | Clone Cells/physiology | - |
dc.subject.MESH | Dental Implantation, Endosseous/methods* | - |
dc.subject.MESH | Feasibility Studies | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Mutant Strains | - |
dc.subject.MESH | Middle Aged | - |
dc.subject.MESH | Osteoblasts/physiology | - |
dc.subject.MESH | Osteogenesis/physiology | - |
dc.subject.MESH | Osteotomy/methods* | - |
dc.subject.MESH | Stromal Cells/physiology* | - |
dc.subject.MESH | Tissue Engineering* | - |
dc.subject.MESH | Transplantation, Heterologous | - |
dc.title | Acquisition of human alveolar bone-derived stromal cells using minimally irrigated implant osteotomy: in vitro and in vivo evaluations | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Periodontology (치주과학) | - |
dc.contributor.googleauthor | Jung-Chul Park | - |
dc.contributor.googleauthor | Jane C. Kim | - |
dc.contributor.googleauthor | Yong-Tae Kim | - |
dc.contributor.googleauthor | Seong-Ho Choi | - |
dc.contributor.googleauthor | Kyoo-Sung Cho | - |
dc.contributor.googleauthor | Gun-Il Im | - |
dc.contributor.googleauthor | Byung-Soo Kim | - |
dc.contributor.googleauthor | Chang-Sung Kim | - |
dc.identifier.doi | 22420633 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01041 | - |
dc.contributor.localId | A03810 | - |
dc.contributor.localId | A01653 | - |
dc.contributor.localId | A04081 | - |
dc.relation.journalcode | J01337 | - |
dc.identifier.eissn | 1600-051X | - |
dc.identifier.pmid | 22420633 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/j.1600-051X.2012.01865.x/abstract | - |
dc.subject.keyword | dental implant | - |
dc.subject.keyword | human bone marrow stromal cells | - |
dc.subject.keyword | tissue engineering | - |
dc.contributor.alternativeName | Kim, Chang Sung | - |
dc.contributor.alternativeName | Cho, Kyoo Sung | - |
dc.contributor.alternativeName | Choi, Seong Ho | - |
dc.contributor.alternativeName | Park, Jung Chul | - |
dc.contributor.affiliatedAuthor | Kim, Chang Sung | - |
dc.contributor.affiliatedAuthor | Cho, Kyoo Sung | - |
dc.contributor.affiliatedAuthor | Park, Jung Chul | - |
dc.contributor.affiliatedAuthor | Choi, Seong Ho | - |
dc.citation.volume | 39 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 495 | - |
dc.citation.endPage | 505 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CLINICAL PERIODONTOLOGY, Vol.39(5) : 495-505, 2012 | - |
dc.identifier.rimsid | 33482 | - |
dc.type.rims | ART | - |
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