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miR-200b regulates cell migration via Zeb family during mouse palate development.

Authors
 Jeong-Oh Shin  ;  Eizo Nakagawa  ;  Eun-Jung Kim  ;  Kyoung-Won Cho  ;  Jong-Min Lee  ;  Sung-Won Cho  ;  Han-Sung Jung 
Citation
 HISTOCHEMISTRY AND CELL BIOLOGY, Vol.137(4) : 459-470, 2012 
Journal Title
 HISTOCHEMISTRY AND CELL BIOLOGY 
ISSN
 0948-6143 
Issue Date
2012
MeSH
Animals ; Cadherins/genetics ; Cadherins/metabolism ; Cell Movement* ; Cells, Cultured ; Epithelial-Mesenchymal Transition ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism* ; Kruppel-Like Transcription Factors/genetics ; Kruppel-Like Transcription Factors/metabolism* ; Mice ; Mice, Inbred ICR ; MicroRNAs/genetics ; MicroRNAs/metabolism* ; Palate/embryology* ; Palate/metabolism* ; Repressor Proteins/genetics ; Repressor Proteins/metabolism* ; Zinc Finger E-box Binding Homeobox 2 ; Zinc Finger E-box-Binding Homeobox 1
Keywords
miR-200b ; Zeb1 ; Zeb2 ; E-cadherin ; Cell migration ; Palatal fusion
Abstract
Palate development requires coordinating proper cellular and molecular events in palatogenesis, including the epithelial-mesenchymal transition (EMT), apoptosis, cell proliferation, and cell migration. Zeb1 and Zeb2 regulate epithelial cadherin (E-cadherin) and EMT during organogenesis. While microRNA 200b (miR-200b) is known to be a negative regulator of Zeb1 and Zeb2 in cancer progression, its regulatory effects on Zeb1 and Zeb2 in palatogenesis have not yet been clarified. The aim of this study is to investigate the relationship between the regulators of palatal development, specifically, miR-200b and the Zeb family. Expression of both Zeb1 and Zeb2 was detected in the mesenchyme of the mouse palate, while miR-200b was expressed in the medial edge epithelium. After contact with the palatal shelves, miR-200b was expressed in the palatal epithelial lining and epithelial island around the fusion region but not in the palatal mesenchyme. The function of miR-200b was examined by overexpression via a lentiviral vector in the palatal shelves. Ectopic expression of miR-200b resulted in suppression of the Zeb family, upregulation of E-cadherin, and changes in cell migration and palatal fusion. These results suggest that miR-200b plays crucial roles in cell migration and palatal fusion by regulating Zeb1 and Zeb2 as a noncoding RNA during palate development.
Full Text
http://link.springer.com/article/10.1007%2Fs00418-012-0915-6
DOI
22261924
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eun-Jung(김은정) ORCID logo https://orcid.org/0000-0002-9515-7590
Nakagawa, Eizo(나까가와에이죠)
Shin, Jeong Oh(신정오) ORCID logo https://orcid.org/0000-0002-6935-0936
Lee, Jong Min(이종민) ORCID logo https://orcid.org/0000-0002-9466-7644
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
Cho, Kyoung Won(조경원)
Cho, Sung Won(조성원) ORCID logo https://orcid.org/0000-0001-7505-9769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/90371
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