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The novel function of Oct3/4 in mouse tooth development.

Authors
 Eizo Nakagawa  ;  Li Zhang  ;  Eun-Jung Kim  ;  Jeong-Oh Shin  ;  Sung-Won Cho  ;  Hayato Ohshima  ;  Han-Sung Jung 
Citation
 HISTOCHEMISTRY AND CELL BIOLOGY, Vol.137(3) : 367-376, 2012 
Journal Title
 HISTOCHEMISTRY AND CELL BIOLOGY 
ISSN
 0948-6143 
Issue Date
2012
MeSH
Animals ; Apoptosis/physiology ; Cell Differentiation/physiology ; Cell Nucleus/metabolism ; Cytoplasm/metabolism ; Female ; Gene Expression Regulation, Developmental/physiology* ; Gene Knockdown Techniques ; Mice ; Mice, Inbred ICR ; Octamer Transcription Factor-3/genetics* ; Octamer Transcription Factor-3/metabolism ; Odontogenesis/physiology* ; Organ Culture Techniques ; Pregnancy ; Tooth*/cytology ; Tooth*/embryology ; Tooth*/physiology
Keywords
Oct3/4 ; Isoforms ; Apoptosis ; Tooth morphogenesis
Abstract
Octamer-binding factor 3/4 (Oct3/4) is one of the key regulators maintaining the pluripotency and self-renewal in embryonic stem cells and is involved in the developmental events. However, the functional significance of Oct3/4 remains to be clarified during tooth morphogenesis. This study aimed to examine the functional role of Oct3/4 in mouse. During tooth morphogenesis (E11-E16.5), Oct3/4-positive cells, detected by nuclear immunoreaction, increased in number, and subsequently, their immunoreaction shifted from the nucleus to the cytoplasm at the stage of cell differentiation (E18.5). Quantitative real-time PCR clearly demonstrated the relationship between isoforms of Oct3/4 and the in vivo cellular localization of Oct3/4, suggesting that the Oct3/4 expressed in nucleus was Oct3/4A, whereas that expressed in the cytoplasm was Oct3/4B. RNAi knockdown of Oct3/4 induced apoptosis and arrested tooth morphogenesis. Our results suggest that (1) the increased number of Oct3/4-positive cells with nuclear immunoreaction correlate with active cell proliferation during tooth morphogenesis and (2) the shift of Oct3/4 from the nucleus to the cytoplasm plays a crucial role in cell differentiation.
Full Text
http://link.springer.com/article/10.1007%2Fs00418-011-0895-y
DOI
22159899
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eun-Jung(김은정) ORCID logo https://orcid.org/0000-0002-9515-7590
Nakagawa, Eizo(나까가와에이죠)
Shin, Jeong Oh(신정오) ORCID logo https://orcid.org/0000-0002-6935-0936
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
Cho, Sung Won(조성원) ORCID logo https://orcid.org/0000-0001-7505-9769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/90370
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