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Methylation status of lamin A/C in gastric cancer cell lines.

Authors
 Lee WS  ;  Jung JJ  ;  Jeung HC  ;  Noh SW  ;  Oh BK  ;  Kim KY  ;  Kim TS  ;  Chung HC  ;  Roh JK  ;  Rha SY 
Citation
 HEPATO-GASTROENTEROLOGY, Vol.59(116) : 1313-1318, 2012 
Journal Title
HEPATO-GASTROENTEROLOGY
ISSN
 0172-6390 
Issue Date
2012
MeSH
Cell Line, Tumor ; Colorectal Neoplasms/genetics ; CpG Islands ; DNA Methylation* ; Down-Regulation ; Humans ; Lamin Type A/genetics* ; Lamin Type A/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Stomach Neoplasms/genetics* ; Stomach Neoplasms/metabolism
Keywords
Cell Line, Tumor ; Colorectal Neoplasms/genetics ; CpG Islands ; DNA Methylation* ; Down-Regulation ; Humans ; Lamin Type A/genetics* ; Lamin Type A/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Stomach Neoplasms/genetics* ; Stomach Neoplasms/metabolism
Abstract
BACKGROUND/AIMS: Epigenetic regulations play a role in the development and progression of cancer. Therefore, discovering novel epigenetically regulated genes could provide useful information in understanding cancer. Lamin A/C is an intermediate filament protein whose expression is reported to be suppressed in tissues of gastro-intestinal malignancies. We examined expression of lamin A/C in gastric and colorectal cancer cell lines and its association with DNA methylation.

METHODOLOGY: The methylation status of CpG island in 19 gastric, 5 colorectal cancer cells and 1 normal colon cell line were examined with methylation-specific PCR using paired methylated and unmethylated primers. The level of mRNA expression of lamin A/C was detected using RT-PCR.

RESULTS: Eighteen gastric cancer cell lines showed 95% unmethylation of lamin A/C and 1 cell line showed partial methylation. In colorectal cancer, only 1 out of 5 cancer cell lines (20%) was partially methylated and the remaining cell lines, including 1 normal colon cell line was unmethylated. With RT-PCR, all cell lines demonstrated mRNA expression of lamin A/C regardless of methylation status.

CONCLUSIONS: We observed that the expression of lamin A/C was not suppressed in gastrointestinal cancer cell lines different from hematologic malignant cells and it is not regulated through DNA methylation.
DOI
22057372
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Noh, Se Won(노세원)
Roh, Jae Kyung(노재경)
Rha, Sun Young(라선영) ORCID logo https://orcid.org/0000-0002-2512-4531
Oh, Bong Kyeong(오봉경)
Lee, Won Suk(이원석)
Chung, Hyun Cheol(정현철) ORCID logo https://orcid.org/0000-0002-0920-9471
Jeung, Hei Cheul(정희철) ORCID logo https://orcid.org/0000-0003-0952-3679
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/90353
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