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Methylation status of lamin A/C in gastric cancer cell lines.
DC Field | Value | Language |
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dc.contributor.author | 노세원 | - |
dc.contributor.author | 노재경 | - |
dc.contributor.author | 라선영 | - |
dc.contributor.author | 오봉경 | - |
dc.contributor.author | 이원석 | - |
dc.contributor.author | 정재준 | - |
dc.contributor.author | 정현철 | - |
dc.contributor.author | 정희철 | - |
dc.date.accessioned | 2014-12-19T16:52:20Z | - |
dc.date.available | 2014-12-19T16:52:20Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0172-6390 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/90353 | - |
dc.description.abstract | BACKGROUND/AIMS: Epigenetic regulations play a role in the development and progression of cancer. Therefore, discovering novel epigenetically regulated genes could provide useful information in understanding cancer. Lamin A/C is an intermediate filament protein whose expression is reported to be suppressed in tissues of gastro-intestinal malignancies. We examined expression of lamin A/C in gastric and colorectal cancer cell lines and its association with DNA methylation. METHODOLOGY: The methylation status of CpG island in 19 gastric, 5 colorectal cancer cells and 1 normal colon cell line were examined with methylation-specific PCR using paired methylated and unmethylated primers. The level of mRNA expression of lamin A/C was detected using RT-PCR. RESULTS: Eighteen gastric cancer cell lines showed 95% unmethylation of lamin A/C and 1 cell line showed partial methylation. In colorectal cancer, only 1 out of 5 cancer cell lines (20%) was partially methylated and the remaining cell lines, including 1 normal colon cell line was unmethylated. With RT-PCR, all cell lines demonstrated mRNA expression of lamin A/C regardless of methylation status. CONCLUSIONS: We observed that the expression of lamin A/C was not suppressed in gastrointestinal cancer cell lines different from hematologic malignant cells and it is not regulated through DNA methylation. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | HEPATO-GASTROENTEROLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Colorectal Neoplasms/genetics | - |
dc.subject.MESH | CpG Islands | - |
dc.subject.MESH | DNA Methylation* | - |
dc.subject.MESH | Down-Regulation | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Lamin Type A/genetics* | - |
dc.subject.MESH | Lamin Type A/metabolism | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Stomach Neoplasms/genetics* | - |
dc.subject.MESH | Stomach Neoplasms/metabolism | - |
dc.title | Methylation status of lamin A/C in gastric cancer cell lines. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Lee WS | - |
dc.contributor.googleauthor | Jung JJ | - |
dc.contributor.googleauthor | Jeung HC | - |
dc.contributor.googleauthor | Noh SW | - |
dc.contributor.googleauthor | Oh BK | - |
dc.contributor.googleauthor | Kim KY | - |
dc.contributor.googleauthor | Kim TS | - |
dc.contributor.googleauthor | Chung HC | - |
dc.contributor.googleauthor | Roh JK | - |
dc.contributor.googleauthor | Rha SY | - |
dc.identifier.doi | 22057372 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01282 | - |
dc.contributor.localId | A01290 | - |
dc.contributor.localId | A02369 | - |
dc.contributor.localId | A03001 | - |
dc.contributor.localId | A03773 | - |
dc.contributor.localId | A03794 | - |
dc.contributor.localId | A01316 | - |
dc.relation.journalcode | J00984 | - |
dc.identifier.pmid | 22057372 | - |
dc.subject.keyword | Cell Line, Tumor | - |
dc.subject.keyword | Colorectal Neoplasms/genetics | - |
dc.subject.keyword | CpG Islands | - |
dc.subject.keyword | DNA Methylation* | - |
dc.subject.keyword | Down-Regulation | - |
dc.subject.keyword | Humans | - |
dc.subject.keyword | Lamin Type A/genetics* | - |
dc.subject.keyword | Lamin Type A/metabolism | - |
dc.subject.keyword | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.keyword | Stomach Neoplasms/genetics* | - |
dc.subject.keyword | Stomach Neoplasms/metabolism | - |
dc.contributor.alternativeName | Noh, Se Won | - |
dc.contributor.alternativeName | Roh, Jae Kyung | - |
dc.contributor.alternativeName | Rha, Sun Young | - |
dc.contributor.alternativeName | Oh, Bong Kyeong | - |
dc.contributor.alternativeName | Lee, Won Suk | - |
dc.contributor.alternativeName | Jung, Jae Joon | - |
dc.contributor.alternativeName | Chung, Hyun Cheol | - |
dc.contributor.alternativeName | Jeung, Hei Cheul | - |
dc.contributor.affiliatedAuthor | Noh, Se Won | - |
dc.contributor.affiliatedAuthor | Roh, Jae Kyung | - |
dc.contributor.affiliatedAuthor | Oh, Bong Kyeong | - |
dc.contributor.affiliatedAuthor | Lee, Won Suk | - |
dc.contributor.affiliatedAuthor | Chung, Hyun Cheol | - |
dc.contributor.affiliatedAuthor | Jeung, Hei Cheul | - |
dc.contributor.affiliatedAuthor | Rha, Sun Young | - |
dc.citation.volume | 59 | - |
dc.citation.number | 116 | - |
dc.citation.startPage | 1313 | - |
dc.citation.endPage | 1318 | - |
dc.identifier.bibliographicCitation | HEPATO-GASTROENTEROLOGY, Vol.59(116) : 1313-1318, 2012 | - |
dc.identifier.rimsid | 34161 | - |
dc.type.rims | ART | - |
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