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Role of heat shock protein 47 in transdifferentiation of human tenon's fibroblasts to myofibroblasts.

Authors
 Samin Hong  ;  Kyoungsoo Park  ;  Jin Hyoung Kim  ;  Sueng-Han Han  ;  Jong Bok Lee  ;  Gong Je Seong 
Citation
 BMC Ophthalmology, Vol.12 : 49, 2012 
Journal Title
 BMC Ophthalmology 
ISSN
 1471-2415 
Issue Date
2012
MeSH
Blotting, Western ; Cell Transdifferentiation/physiology* ; Cells, Cultured ; Fibroblasts/cytology* ; Fibroblasts/metabolism ; Gene Expression Regulation* ; HSP47 Heat-Shock Proteins/physiology* ; Humans ; Myofibroblasts/cytology* ; Myofibroblasts/metabolism ; RNA, Messenger/genetics* ; Real-Time Polymerase Chain Reaction
Keywords
Fibroblast ; Fibrosis ; Heat shock protein ; Myofibroblast ; Transforming growth factor-β
Abstract
BACKGROUND: Heat shock protein 47 (Hsp47) is a well-known molecular chaperone in collagen synthesis and maturation. The aim of this study is to investigate its putative role in the transdifferentiation of Tenon's fibroblasts to myofibroblasts. METHODS: Primary cultured human Tenon's fibroblasts were exposed to transforming growth factor-β1 (TGF-β1) for up to 48 hours. The mRNA levels of Hsp47 and α smooth muscle actin (αSMA) were determined by quantitative real time RT-PCR. After delivery of small interfering RNA (siRNA) molecules targeting Hsp47 into the cells, the expression of Hsp47 and αSMA proteins was determined by western immunoblotting. RESULTS: TGF-β1 increased the mRNA expressions of both Hsp47 and αSMA in human Tenon's fibroblasts, as determined by quantitative real time RT-PCR. However, it induced the protein expression of only αSMA but not Hsp47, as determined by western immunoblots. When siRNAs specific for Hsp47 were introduced into those cells, the TGF-β1-induced expression of αSMA was significantly attenuated on western immunoblots; after 48 hours of exposure to TGF-β1, the relative densities of immunobands were 11.58 for the TGF-β1 only group and 2.75 for the siRNA treatment group, compared with the no treatment control group (p < 0.001). CONCLUSIONS: Our data suggest that Hsp47 may be related to the TGF-β1-induced transdifferentiation of human Tenon's fibroblasts to myofibroblasts.
Files in This Item:
T201203818.pdf Download
DOI
22967132
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Park, Kyungsoo(박경수) ORCID logo https://orcid.org/0000-0002-6972-1143
Seong, Gong Je(성공제) ORCID logo https://orcid.org/0000-0002-5456-4296
Lee, Jong Bok(이종복)
Han, Seung Han(한승한) ORCID logo https://orcid.org/0000-0001-8972-4790
Hong, Sa Min(홍사민)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/89795
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